Validation Data Gallery
|Positive WB detected in||MOLT-4 cells, human peripheral blood leukocyte|
|Positive IHC detected in||human tonsillitis tissue, human appendicitis tissue, human pancreas cancer tissue, human colon cancer tissue, human breast cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:4000-1:20000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66868-1-Ig targets CD8 in WB, IHC, IF applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD8 fusion protein Ag11111|
|Full Name||CD8a molecule|
|Calculated molecular weight||235 aa, 26 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC025715|
|Gene ID (NCBI)||925|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.
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