CD8 Monoclonal antibody
CD8 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF, FC, Cell treatment, ELISA
Conjugate
Unconjugated
CloneNo.
1G2B10
Cat no : 66868-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MOLT-4 cells, human peripheral blood leukocyte |
| Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue, human pancreas cancer tissue, human colon cancer tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
| IHC | See 64 publications below |
| IF | See 29 publications below |
| FC | See 2 publications below |
Product Information
66868-1-Ig targets CD8 in WB, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD8 fusion protein Ag11111 |
| Full Name | CD8a molecule |
| Calculated molecular weight | 235 aa, 26 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC025715 |
| Gene symbol | CD8A |
| Gene ID (NCBI) | 925 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD8 antibody 66868-1-Ig | Download protocol |
| IHC protocol for CD8 antibody 66868-1-Ig | Download protocol |
| IF protocol for CD8 antibody 66868-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Interleukin-10 induces expression of CD39 on CD8+T cells to potentiate anti-PD1 efficacy in EGFR-mutated non-small cell lung cancer | ||
Nat Commun Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma. | ||
Mol Ther Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes. | ||
Brief Bioinform Machine learning-based tumor-infiltrating immune cell-associated lncRNAs for predicting prognosis and immunotherapy response in patients with glioblastoma | ||
Front Immunol LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma | ||
Front Immunol A novel prognostic signatures based on metastasis- and immune-related gene pairs for colorectal cancer |
