CD8 Monoclonal antibody

CD8 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

1G2B10

Cat no : 66868-1-Ig

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32



Tested Applications

Positive WB detected inMOLT-4 cells, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human pancreas cancer tissue, human colon cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD8 Ready-To-Use IHC Kit. CD8 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:4000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66868-1-Ig targets CD8 in WB, IHC, IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD8 fusion protein Ag11111
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

Protocols

Product Specific Protocols
WB protocol for CD8 antibody 66868-1-IgDownload protocol
IHC protocol for CD8 antibody 66868-1-IgDownload protocol
IF protocol for CD8 antibody 66868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC,IF

Oncoimmunology

Development of bispecific anti-c-Met/PD-1 diabodies for the treatment of solid tumors and the effect of c-Met binding affinity on efficacy.

Authors - Qingyun Yuan
humanIHC

Viral Immunol

A Comparison of Splenic Pathologic Change and Immune Function in HBV-Related Portal Hypertension and Chinese Budd-Chiari Syndrome Patients with Hypersplenism.

Authors - Suxin Li
humanIHC

Chin Med J (Engl)

Hyperbaric oxygen treatment on keloid tumor immune gene expression.

Authors - Chun-Hu Wang
humanIHC

Onco Targets Ther

Role of GLUT-1 in the Upregulation of PD-L1 Expression After Radiotherapy and Association of PD-L1 with Favourable Overall Survival in Hypopharyngeal Cancer.

Authors - Li-Fang Shen
humanIHC

Int Immunopharmacol

The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data

Authors - Xiaomin Lu
humanWB

Int Immunopharmacol

A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment.

Authors - Shuling Zhang