CD8 Monoclonal antibody
CD8 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF, FC, Cell treatment, ELISA
Conjugate
Unconjugated
CloneNo.
1G2B10
Cat no : 66868-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MOLT-4 cells, human peripheral blood leukocyte |
Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue, human pancreas cancer tissue, human colon cancer tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
IHC | See 64 publications below |
IF | See 29 publications below |
FC | See 2 publications below |
Product Information
66868-1-Ig targets CD8 in WB, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD8 fusion protein Ag11111 |
Full Name | CD8a molecule |
Calculated molecular weight | 235 aa, 26 kDa |
Observed molecular weight | 32 kDa |
GenBank accession number | BC025715 |
Gene symbol | CD8A |
Gene ID (NCBI) | 925 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.
Protocols
Product Specific Protocols | |
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WB protocol for CD8 antibody 66868-1-Ig | Download protocol |
IHC protocol for CD8 antibody 66868-1-Ig | Download protocol |
IF protocol for CD8 antibody 66868-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Immunother Cancer Interleukin-10 induces expression of CD39 on CD8+T cells to potentiate anti-PD1 efficacy in EGFR-mutated non-small cell lung cancer | ||
Nat Commun Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma. | ||
Mol Ther Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes. | ||
Brief Bioinform Machine learning-based tumor-infiltrating immune cell-associated lncRNAs for predicting prognosis and immunotherapy response in patients with glioblastoma | ||
Front Immunol LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma | ||
Front Immunol A novel prognostic signatures based on metastasis- and immune-related gene pairs for colorectal cancer |