Validation Data Gallery
|Positive WB detected in
|MOLT-4 cells, human peripheral blood leukocyte
|Positive IHC detected in
|human tonsillitis tissue, human appendicitis tissue, human pancreas cancer tissue, human colon cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human tonsillitis tissue
|Western Blot (WB)
|WB : 1:2000-1:10000
|IHC : 1:10000-1:40000
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66868-1-Ig targets CD8a in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype
|Mouse / IgG1
|CD8a fusion protein Ag11111
|Calculated molecular weight
|235 aa, 26 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.
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