CD90 Monoclonal antibody

CD90 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rat, rabbit, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D7D11

Cat no : 66766-1-Ig

Synonyms

CD90, CDw90, FLJ33325, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein, THY1



Tested Applications

Positive WB detected infetal human brain tissue, rabbit brain tissue, pig colon tissue, U2OS cells, human kidney tissue, pig brain tissue, pig spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human colon cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66766-1-Ig targets CD90 in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

Tested Reactivity Human, pig, rat, rabbit, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD90 fusion protein Ag25603
Full Name Thy-1 cell surface antigen
Calculated molecular weight 161 aa, 18 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC065559
Gene symbol THY1
Gene ID (NCBI) 7070
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Protocols

Product Specific Protocols
WB protocol for CD90 antibody 66766-1-IgDownload protocol
IHC protocol for CD90 antibody 66766-1-IgDownload protocol
IF protocol for CD90 antibody 66766-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mol Cell Biol

Cancer-associated adipocytes-derived G-CSF promotes breast cancer malignancy via Stat3 signaling.

Authors - Li Liu
humanIHC

Int J Mol Sci

Expression of Cancer Stem Cell Markers EpCAM and CD90 Is Correlated with Anti- and Pro-Oncogenic EphA2 Signaling in Hepatocellular Carcinoma.

Authors - Nobuhiko Asakura

Cell Reprogram

Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.

Authors - Qi Li
humanIF,WB

Cell Signal

miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.

Authors - Dong-Min Shi