|Positive WB detected in||fetal human brain tissue, rabbit brain tissue, pig colon tissue, U2OS cells, human kidney tissue, pig brain tissue, pig spleen tissue|
|Positive IHC detected in||human tonsillitis tissue, human lung cancer tissue, human colon cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66766-1-Ig targets CD90 in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.
|Tested Reactivity||Human, pig, rat, rabbit, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CD90 fusion protein Ag25603|
|Full Name||Thy-1 cell surface antigen|
|Calculated molecular weight||161 aa, 18 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC065559|
|Gene ID (NCBI)||7070|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.
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