CD98/SLC3A2 Monoclonal antibody

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  WB analysis using 66883-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  IHC staining of human renal cell carcinoma using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66883-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD98/SLC3A2 antibody (66883-1-Ig, Clone: 2B10F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CD98/SLC3A2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66883-1-Ig

Clone No.2B10F5

Publications(8)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD98, SLC3A2, 2B10F5, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HepG2 cells, HT-29 cells, COLO 320 cells, Caco-2 cells, Jurkat cells
• Positive IHC detected in: human renal cell carcinoma tissue, human colon cancer tissue, human cervical cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below

Product Information

66883-1-Ig targets CD98/SLC3A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7301

  Product name: Recombinant human SLC3A2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 269-630 aa of BC001061

  Sequence: KDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

• Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 85-94 kDa, 120-130 kDa
• GenBank Accession Number: BC001061
• Gene Symbol: CD98
• Gene ID (NCBI): 6520
• RRID: AB_2882215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08195
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Extracell Vesicles; Homosalate boosts the release of tumour-derived extracellular vesicles with protection against anchorage-loss property.; Authors - Eleonora Grisard
human	WB	Nat Commun; Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9.; Authors - Mathilde Mathieu
human	WB	Cancer Cell Int; Matrix stiffness-dependent STEAP3 coordinated with PD-L2 identify tumor responding to sorafenib treatment in hepatocellular carcinoma; Authors - Shunxi Wang
human	WB,IF	Phytomedicine; Identification of the target protein and molecular mechanism of honokiol in anti-inflammatory action; Authors - Xiaoying Cai
human	WB	Oncol Lett; Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2; Authors - Xiangtian Shi
human	WB	Proc Natl Acad Sci U S A; Activation of polyamine catabolism promotes glutamine metabolism and creates a targetable vulnerability in lung cancer; Authors - Xinlu Han