RASGRF1 Polyclonal antibody

RASGRF1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 12958-1-AP

Synonyms

CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RASGRF1



Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IP detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

12958-1-AP targets RASGRF1 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASGRF1 fusion protein Ag4072
Full Name Ras protein-specific guanine nucleotide-releasing factor 1
Calculated molecular weight 134 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC040275
Gene symbol RASGRF1
Gene ID (NCBI) 5923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.

Protocols

Product Specific Protocols
WB protocol for RASGRF1 antibody 12958-1-APDownload protocol
IP protocol for RASGRF1 antibody 12958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Med

Modulation of B-cell receptor and microenvironment signaling by a guanine exchange factor in B-cell malignancies.

Authors - Wei Liao
humanWB

Med Oncol

DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway.

Authors - Wei Huang

Leuk Lymphoma

Amplification of B cell receptor-Erk signaling by Rasgrf-1 over-expression in chronic lymphocytic leukemia.

Authors - Wei Liao
humanWB

Molecules

Ginsenoside PPD's Antitumor Effect via Down-Regulation of mTOR Revealed by Super-Resolution Imaging.

Authors - Bo Teng
mouseWB

J Agric Food Chem

Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.

Authors - Jie Wei