CDC37 Polyclonal antibody

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  WB analysis of HEK-293 using 10218-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10218-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10218-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10218-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 10218-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10218-1-AP

  IP result of anti-CDC37 (IP:10218-1-AP, 4ug; Detection:10218-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

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  IHC staining of human colon cancer using 10218-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10218-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10218-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CDC37 antibody (10218-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of MCF-7 using 10218-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CDC37 antibody 10218-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HEK-293 using 10218-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 10218-1-AP

  Immunofluorescent analysis of A431 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 10218-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDC37 Polyclonal antibody (10218-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

CDC37 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10218-1-AP

Publications(14)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Synonyms

p50Cdc37, Hsp90 co-chaperone Cdc37, N-terminally processed, Hsp90 co-chaperone Cdc37, Hsp90 co chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, COS-7 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells, A431 cells, MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IP: See 3 publications below
• RIP: See 1 publications below

Product Information

10218-1-AP targets CDC37 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC37 fusion protein Ag0267
• Full Name: cell division cycle 37 homolog (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC000083
• Gene Symbol: CDC37
• Gene ID (NCBI): 11140
• RRID: AB_2077735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.

Publications

Species	Application	Title
human	WB	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
human	IHC	Comput Biol Med; Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data; Authors - Zehua Wang
human	WB	Oncogene; Non-canonical roles of PFKFB3 in regulation of cell cycle through binding to CDK4.; Authors - Wenzhi Jia
human	WB	Int J Mol Sci; (20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.; Authors - Chen Chen
human	WB, IP	Pharmacol Res; Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.; Authors - Dongrong Zhu
human	WB	Inflammation; Inhibition of Cdc37 Ameliorates Arthritis in Collagen-Induced Arthritis Rats by Inhibiting Synoviocyte Proliferation and Migration Through the ERK Pathway; Authors - Weiwei Sun ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (02-11-2022)

Very good antibody for IP and western

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colon cancer cells

FH

Kyosuke (Verified Customer) (06-12-2019)

It works good on HEK293T cells for Western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T