Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, COS-7 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells, A431 cells, MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10218-1-AP targets CDC37 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0267 Product name: Recombinant human CDC37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC000083 Sequence: MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQV Predict reactive species |
| Full Name | cell division cycle 37 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC000083 |
| Gene Symbol | CDC37 |
| Gene ID (NCBI) | 11140 |
| RRID | AB_2077735 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16543 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CDC37 antibody 10218-1-AP | Download protocol |
| IF protocol for CDC37 antibody 10218-1-AP | Download protocol |
| IHC protocol for CDC37 antibody 10218-1-AP | Download protocol |
| IP protocol for CDC37 antibody 10218-1-AP | Download protocol |
| WB protocol for CDC37 antibody 10218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDC37 Polyclonal Antibody (Cat# 10218-1-AP) has accumulated 19 citations across journals including Nature Communications, Advanced Science, Oncogene, Pharmacological Research, Phytomedicine, International Journal of Molecular Sciences, iScience, Cell Signaling, Inflammation, and Naunyn-Schmiedeberg's Archives of Pharmacology. Associated research fields span cancer biology (breast, liver, gastric, melanoma, osteosarcoma, NSCLC, AML), Parkinson's disease, arthritis, cell cycle regulation, HSP90–CDC37 co-chaperone signaling, autophagy, and immune response.
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
Adv Sci (Weinh) Tumor-Derived CDC37 Inhibits Antigen Cross-Presentation in Dendritic Cells and Impairs Anti-Tumor Immunity in Breast Cancer. | ||
Adv Sci (Weinh) The FBXW7-RPAP2 Axis Controls the Growth of Hepatocellular Carcinoma Cells and Determines the Fate of Liver Cell Differentiation | ||
Pharmacol Res Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect. | ||
Phytomedicine Celastrol protected the MPTP-injected mice Parkinson's disease model via redox regulation of CDC37. | ||
Oncogene Non-canonical roles of PFKFB3 in regulation of cell cycle through binding to CDK4. |
Reviews
What customers say
All four reviews are positive, with three five-star and one four-star ratings. Users consistently report excellent performance in Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation. Bands are described as precise with clear structure, making downstream analysis straightforward. The antibody works well in colon cancer cells and HEK293T cells. Recommended dilutions range from 1:400 to 1:2000 depending on the application. Overall, reviewers express high satisfaction with specificity and band quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (01-08-2026) | Antibodies are very good, nice work!
|
Mounika (Verified Customer) (01-08-2026) | Bands are very precise, easy in sorting the further steps, wonderful bands structure! happy to them!
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Iram (Verified Customer) (02-11-2022) | Very good antibody for IP and western
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Kyosuke (Verified Customer) (06-12-2019) | It works good on HEK293T cells for Western blot
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