|Positive WB detected in||HEK-293 cells, A431 cells, COS-7 cells, Jurkat cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, A431 cells, HEK-293 cells, MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10218-1-AP targets CDC37 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, monkey, mouse samples.
|Tested Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDC37 fusion protein Ag0267|
|Full Name||cell division cycle 37 homolog (S. cerevisiae)|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC000083|
|Gene ID (NCBI)||11140|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.
|Product Specific Protocols|
|WB protocol for CDC37 antibody 10218-1-AP||Download protocol|
|IHC protocol for CDC37 antibody 10218-1-AP||Download protocol|
|IF protocol for CDC37 antibody 10218-1-AP||Download protocol|
|IP protocol for CDC37 antibody 10218-1-AP||Download protocol|
|FC protocol for CDC37 antibody 10218-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kyosuke (Verified Customer) (06-12-2019)
It works good on HEK293T cells for Western blot