CDC37 Polyclonal antibody
CDC37 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, monkey, mouse
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10218-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, A431 cells, COS-7 cells, Jurkat cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, A431 cells, HEK-293 cells, MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 7 publications below |
IP | See 2 publications below |
Product Information
10218-1-AP targets CDC37 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, monkey, mouse samples.
Tested Reactivity | human, monkey, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CDC37 fusion protein Ag0267 |
Full Name | cell division cycle 37 homolog (S. cerevisiae) |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC000083 |
Gene symbol | CDC37 |
Gene ID (NCBI) | 11140 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CDC37 antibody 10218-1-AP | Download protocol |
IHC protocol for CDC37 antibody 10218-1-AP | Download protocol |
IF protocol for CDC37 antibody 10218-1-AP | Download protocol |
IP protocol for CDC37 antibody 10218-1-AP | Download protocol |
FC protocol for CDC37 antibody 10218-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
Oncogene Non-canonical roles of PFKFB3 in regulation of cell cycle through binding to CDK4. | ||
Pharmacol Res Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect. | ||
Int J Mol Sci Novel HSP90-PI3K Dual Inhibitor Suppresses Melanoma Cell Proliferation by Interfering with HSP90-EGFR Interaction and Downstream Signaling Pathways. | ||
Cells KRAB-Zinc Finger Protein ZNF268a Deficiency Attenuates the Virus-Induced Pro-Inflammatory Response by Preventing IKK Complex Assembly. | ||
Int J Mol Sci (20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iram (Verified Customer) (02-11-2022) | Very good antibody for IP and western
|
FH Kyosuke (Verified Customer) (06-12-2019) | It works good on HEK293T cells for Western blot
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