CDC37 Polyclonal antibody

CDC37 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, mouse

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10218-1-AP

Synonyms

CDC37, CDC37A, Hsp90 co chaperone Cdc37, P50CDC37



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, COS-7 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A431 cells, HEK-293 cells, MCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10218-1-AP targets CDC37 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, monkey, mouse samples.

Tested Reactivity human, monkey, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC37 fusion protein Ag0267
Full Name cell division cycle 37 homolog (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC000083
Gene symbol CDC37
Gene ID (NCBI) 11140
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.

Protocols

Product Specific Protocols
WB protocol for CDC37 antibody 10218-1-APDownload protocol
IHC protocol for CDC37 antibody 10218-1-APDownload protocol
IF protocol for CDC37 antibody 10218-1-APDownload protocol
IP protocol for CDC37 antibody 10218-1-APDownload protocol
FC protocol for CDC37 antibody 10218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Enzyme Inhib Med Chem

Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.

Authors - Feifei Qin
humanWB,IP

Cells

KRAB-Zinc Finger Protein ZNF268a Deficiency Attenuates the Virus-Induced Pro-Inflammatory Response by Preventing IKK Complex Assembly.

Authors - Yi Liu
humanWB

Int J Mol Sci

Novel HSP90-PI3K Dual Inhibitor Suppresses Melanoma Cell Proliferation by Interfering with HSP90-EGFR Interaction and Downstream Signaling Pathways.

Authors - Qian Zhao
humanWB, IP

Pharmacol Res

Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.

Authors - Dongrong Zhu
humanWB

Oncogene

Non-canonical roles of PFKFB3 in regulation of cell cycle through binding to CDK4.

Authors - Wenzhi Jia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

It works good on HEK293T cells for Western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T