CDC42BPB Polyclonal antibody

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  WB analysis using 31526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31526-1-AP (CDC42BPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CDC42BPB Polyclonal Antibody for WB, ELISA

Cat No. 31526-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

CDC42BPB, DMPK like beta, KIAA1124, MRCK beta, MRCKB

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31526-1-AP targets CDC42BPB in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC42BPB fusion protein Ag35745
• Full Name: CDC42 binding protein kinase beta (DMPK-like)
• Calculated Molecular Weight: 194 kDa
• Observed Molecular Weight: 194 kDa
• GenBank Accession Number: NM_006035.3
• Gene Symbol: CDC42BPB
• Gene ID (NCBI): 9578
• RRID: AB_3670021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y5S2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC42BPB is a serine/threonine protein kinase and codes for MRCKβ (myotonic dystrophy-related Cdc42-binding kinase beta), a regulator of cell cytoskeletal reorganization and cell migration. CDC42BPB shows expression in the granule cell layer of the lateral adult cerebellum, which has been associated with cognitive functions, and has recently been associated with neurodevelopmental disorders including ASD (autism spectrum disorder). (PMID: 35942939)