CDCP1 Polyclonal antibody

CDCP1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12754-1-AP

Synonyms

CD318, CDCP1, Membrane glycoprotein gp140, SIMA135, TRASK

---

Validation Data Gallery

• 

  WB analysis using 12754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12754-1-AP (CDCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12754-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12754-1-AP (CDCP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A549 using 12754-1-AP

  1X10^6 A549 cells were stained with 0.2ug CDCP1 antibody (12754-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, PC-3 cells
• Positive FC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12754-1-AP targets CDCP1 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDCP1 fusion protein Ag3439
• Full Name: CUB domain containing protein 1
• Calculated Molecular Weight: 836 aa, 93 kDa
• Observed Molecular Weight: 72 kDa, 130 kDa
• GenBank Accession Number: BC021099
• Gene Symbol: CDCP1
• Gene ID (NCBI): 64866
• RRID: AB_2078799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCP1, also named as CD318, TRASK and SIMA 135, is a 135-140 kDa transmembrane protein that contains three CUB domains within the extracellular region and a hexalysine stretch within the cytoplasmic region. A soluble form with MW 80 kDa (p80) is cleaved at an unidentified site on the N-terminal side. CDCP1 is a novel marker for hematopoietic stem cells.

Publications

Species	Application	Title
mouse	WB	Theranostics; Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity.; Authors - Wanzun Lin
human	WB	Aging (Albany NY); The emerging role of the MiR-1272-ADAM9-CDCP1 signaling pathway in the progression of glioma.; Authors - Fei Geng