CDCP1 Polyclonal antibody

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  WB analysis using 12754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12754-1-AP (CDCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12754-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12754-1-AP (CDCP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 12754-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12754-1-AP (CDCP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12754-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12754-1-AP (CDCP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CDCP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12754-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Transmembrane and associated with src kinases, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, PC-3 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12754-1-AP targets CDCP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDCP1 fusion protein Ag3439
• Full Name: CUB domain containing protein 1
• Calculated Molecular Weight: 836 aa, 93 kDa
• Observed Molecular Weight: 72 kDa, 130 kDa
• GenBank Accession Number: BC021099
• Gene Symbol: CDCP1
• Gene ID (NCBI): 64866
• RRID: AB_2078799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H5V8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCP1, also called CD318, TRASK, and SIMA 135, is a 135-140 kDa transmembrane protein containing three CUB domains within the extracellular region and a hexalysine stretch within the cytoplasmic region. A soluble form with MW 80 kDa (p80) is cleaved at an unidentified site on the N-terminal side. CDCP1 is a novel marker for hematopoietic stem cells.

Publications

Species	Application	Title
mouse	WB	Theranostics; Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity.; Authors - Wanzun Lin
human	WB	Aging (Albany NY); The emerging role of the MiR-1272-ADAM9-CDCP1 signaling pathway in the progression of glioma.; Authors - Fei Geng