CDK4 Monoclonal antibody

CDK4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G2C12

Cat no : 66950-1-Ig

Synonyms

CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

66950-1-Ig targets CDK4 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDK4 fusion protein Ag20538
Full Name cyclin-dependent kinase 4
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC010153
Gene symbol CDK4
Gene ID (NCBI) 1019
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).

Protocols

Product Specific Protocols
WB protocol for CDK4 antibody 66950-1-IgDownload protocol
IHC protocol for CDK4 antibody 66950-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biol Pharm Bull

Discovery and Verification of Key Liver Cancer Genes and Alternative Splicing Events Based on Second-Generation Sequencing Data Analysis.

Authors - Mengqi Cui
humanWB

J Cancer

PSMD7 downregulation suppresses lung cancer progression by regulating the p53 pathway.

Authors - Xinchun Xu
humanWB

Mol Cell Endocrinol

Long noncoding RNA GAS5 accelerates diabetic wound healing and promotes lymphangiogenesis via miR-217/Prox1 axis.

Authors - Zhi-You He
humanWB

Int J Oncol

GBP1 promotes erlotinib resistance via PGK1‑activated EMT signaling in non‑small cell lung cancer.

Authors - Lifang Cheng
humanWB

Front Cell Dev Biol

NLGN3 Upregulates Expression of ADAM10 to Promote the Cleavage of NLGN3 via Activating the LYN Pathway in Human Gliomas.

Authors - Ning-Ning Dang
mouseWB

Front Cell Dev Biol

LHPP-Mediated Histidine Dephosphorylation Suppresses the Self-Renewal of Mouse Embryonic Stem Cells.

Authors - Rong Mu Xia