Validation Data Gallery
|Positive WB detected in||LNCaP cells, HeLa cells, HEK-293 cells, 4T1 cells, Jurkat cells, MCF-7 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells|
|Positive IHC detected in||human breast cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Sample-dependent, check data in validation data gallery|
66950-1-Ig targets CDK4 in WB, IHC, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CDK4 fusion protein Ag20538|
|Full Name||cyclin-dependent kinase 4|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC010153|
|Gene ID (NCBI)||1019|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).
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