CEP290 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP290 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 22490-1-AP

Synonyms

CT87, Centrosomal protein of 290 kDa, Cancer/testis antigen 87, BBS14, Bardet-Biedl syndrome 14 protein

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Validation Data Gallery

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  WB analysis of HeLa using 22490-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22490-1-AP (CEP290 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22490-1-AP

  IP result of anti-CEP290 (IP:22490-1-AP, 4ug; Detection:22490-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IHC staining of human prostate cancer using 22490-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 22490-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22490-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 22490-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CEP290 antibody (22490-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

22490-1-AP targets CEP290 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP290 fusion protein Ag17457
• Full Name: centrosomal protein 290kDa
• Calculated Molecular Weight: 2479 aa, 290 kDa
• Observed Molecular Weight: 290 kDa
• GenBank Accession Number: BC008641
• Gene Symbol: CEP290
• Gene ID (NCBI): 80184
• RRID: AB_10973679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. Catalog#22490-1-AP can recognise both.

Publications

Species	Application	Title
mouse	IF	J Cell Biol; The small GTPase RSG1 controls a final step in primary cilia initiation.; Authors - Stephanie O Agbu
mouse	WB	Cell Rep; A CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration.; Authors - Suddhasil Mookherjee
human	IF	EMBO Rep; Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.; Authors - Andrea Martello
human	WB,IHC,IP	Aging (Albany NY); Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway.; Authors - Yiru Shan ; KD Validated
human	WB,IF	J Biol Chem; DAZ interacting protein 1 (Dzip1) phosphorylation by Polo-like kinase 1 (Plk1) regulates the centriolar satellites localization of the BBSome during the cell cycle.; Authors - Boyan Zhang
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jay (Verified Customer) (11-08-2019)

Human RPE1 cell line with Methanol fixation.Immunostaining result shows that CEP290 localizes to centrosome (Red; CEP290, Green; CEP164 for Centrosome marker, Blue;DAPI for Nucleus).The antibody seems to detect additional nucleoli signals.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human RPE1

CEP290 Antibody Immunofluorescence, validation (1:100 dilution) in Human RPE1 (Cat no:22490-1-AP)