Complement factor B Polyclonal antibody

Complement factor B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10170-1-AP

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B



Tested Applications

Positive WB detected inhuman plasma, human blood, rat plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10170-1-AP targets Complement factor B in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag0226
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93-100 kDa, 60-69 kDa
GenBank accession numberBC007990
Gene symbol CFB
Gene ID (NCBI) 629
RRIDAB_2082384
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

Protocols

Product Specific Protocols
WB protocol for Complement factor B antibody 10170-1-APDownload protocol
IHC protocol for Complement factor B antibody 10170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Nat Commun

The classical pathway triggers pathogenic complement activation in membranous nephropathy

Authors - Larissa Seifert
humanWB

J Cell Physiol

IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.

Authors - Xinxuan Cheng
humanWB,IHC,IF

Cell Oncol (Dordr)

Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.

Authors - Reiri Shimazaki
humanWB

Br J Cancer

Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.

Authors - Yanxia Jin
human,mouseWB

Nephrol Dial Transplant

Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation.

Authors - Ming Wu
WB

Chem Res Toxicol

4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.

Authors - Mikhail Linetsky

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

mark (Verified Customer) (03-30-2020)

worked with colourmetric immunohistochemistry

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: E13.5 to E18.5 embryo