Complement factor B Polyclonal antibody

Complement factor B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10170-1-AP

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B



Tested Applications

Positive WB detected inhuman serum tissue, human plasma, human blood
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10170-1-AP targets Complement factor B in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag0226
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93-100 kDa, 60-69 kDa
GenBank accession numberBC007990
Gene symbol CFB
Gene ID (NCBI) 629
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

Protocols

Product Specific Protocols
WB protocol for Complement factor B antibody 10170-1-APDownload protocol
IHC protocol for Complement factor B antibody 10170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.

Authors - Ping Zhao

Am J Physiol Renal Physiol

The C-terminal tail of polycystin-1 regulates complement factor B expression by Signal Transducer and Activator of Transcription 1.

Authors - Ming Wu
WB

Chem Res Toxicol

4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.

Authors - Mikhail Linetsky
humanWB

Br J Cancer

Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.

Authors - Yanxia Jin
humanWB,IHC,IF

Cell Oncol (Dordr)

Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.

Authors - Reiri Shimazaki
humanWB

J Cell Physiol

IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.

Authors - Xinxuan Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

mark (Verified Customer) (03-30-2020)

worked with colourmetric immunohistochemistry

  • Applications: Immunohistochemistry,Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: E13.5 to E18.5 embryo