|Positive WB detected in||human serum tissue, human plasma, human blood|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10170-1-AP targets Complement factor B in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Complement factor B fusion protein Ag0226|
|Full Name||complement factor B|
|Calculated molecular weight||86 kDa|
|Observed molecular weight||93-100 kDa, 60-69 kDa|
|GenBank accession number||BC007990|
|Gene ID (NCBI)||629|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.
The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.
Am J Physiol Renal Physiol
The C-terminal tail of polycystin-1 regulates complement factor B expression by Signal Transducer and Activator of Transcription 1.
Chem Res Toxicol
4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.
Br J Cancer
Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.
Cell Oncol (Dordr)
Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.
J Cell Physiol
IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.
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mark (Verified Customer) (03-30-2020)
worked with colourmetric immunohistochemistry