|Positive WB detected in
|human plasma, human blood, rat plasma
|Positive IHC detected in
|human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:8000
|IHC : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10170-1-AP targets Complement factor B in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
|Host / Isotype
|Rabbit / IgG
|Complement factor B fusion protein Ag0226
|complement factor B
|Calculated molecular weight
|Observed molecular weight
|93-100 kDa, 60-69 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.
The classical pathway triggers pathogenic complement activation in membranous nephropathy
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IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.
Cell Oncol (Dordr)
Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.
Br J Cancer
Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.
Nephrol Dial Transplant
Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation.
Chem Res Toxicol
4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.
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mark (Verified Customer) (03-30-2020)
worked with colourmetric immunohistochemistry