|Positive WB detected in||human serum tissue, human plasma, human blood|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10170-1-AP targets Complement factor B in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Complement factor B fusion protein Ag0226|
|Full Name||complement factor B|
|Calculated molecular weight||86 kDa|
|Observed molecular weight||93-100 kDa, 60-69 kDa|
|GenBank accession number||BC007990|
|Gene ID (NCBI)||629|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.
The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.
Am J Physiol Renal Physiol
The C-terminal tail of polycystin-1 regulates complement factor B expression by Signal Transducer and Activator of Transcription 1.
Chem Res Toxicol
4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.
Br J Cancer
Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.
Cell Oncol (Dordr)
Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.
J Cell Physiol
IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
mark (Verified Customer) (03-30-2020)
worked with colourmetric immunohistochemistry