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Cofilin Monoclonal antibody
Cofilin Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66057-1-Ig
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, PC-12 cells, Neuro-2a cells, pig brain, rat brain, mouse brain, rabbit brain, SH-SY5Y cells, A431 cells|
|Positive IHC detected in||human testis tissue, human lung cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66057-1-Ig is Cofilin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig, rabbit|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cofilin fusion protein Ag18044|
|Full Name||cofilin 1 (non-muscle)|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC012318|
|Gene ID (NCBI)||1072|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.
Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.
Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.
Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A
Int J Nanomedicine
Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.
Hum Mol Genet
Neuroligin-3 and Neuroligin-4X form Nanoscopic clusters and regulate growth cone organization and size.