- Featured Product
- KD/KO Validated
Cofilin Monoclonal antibody
Cofilin Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1G6A2
Cat no : 66057-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-12 cells, Neuro-2a cells, pig brain, rat brain, mouse brain, rabbit brain, SH-SY5Y cells, A431 cells |
| Positive IHC detected in | human testis tissue, human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 66057-1-Ig is Cofilin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cofilin fusion protein Ag18044 |
| Full Name | cofilin 1 (non-muscle) |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC012318 |
| Gene symbol | CFL1 |
| Gene ID (NCBI) | 1072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cofilin antibody 66057-1-Ig | Download protocol |
| IHC protocol for Cofilin antibody 66057-1-Ig | Download protocol |
| IF protocol for Cofilin antibody 66057-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Psychiatry Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning. | ||
Nat Commun Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion. | ||
Nat Commun Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice. | ||
Elife Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A | ||
Int J Nanomedicine Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity. | ||
Hum Mol Genet Neuroligin-3 and Neuroligin-4X form Nanoscopic clusters and regulate growth cone organization and size. |
