Cofilin Monoclonal antibody

• Featured Product
• KD/KO Validated

Cofilin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

1G6A2

Cat no : 66057-1-Ig

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18

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Validation Data Gallery

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  WB analysis using 66057-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66057-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66057-1-Ig

  Immunofluorescent analysis of Hela cells using 66057-1-Ig (Cofilin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-12 cells, Neuro-2a cells, pig brain, rat brain, mouse brain, rabbit brain, SH-SY5Y cells, A431 cells
• Positive IHC detected in: human testis tissue, human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 1 publications below

Product Information

The immunogen of 66057-1-Ig is Cofilin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cofilin fusion protein Ag18044
• Full Name: cofilin 1 (non-muscle)
• Calculated molecular weight: 18 kDa
• Observed molecular weight: 18 kDa
• GenBank accession number: BC012318
• Gene symbol: CFL1
• Gene ID (NCBI): 1072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Neurosci; C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.; Authors - Rajeeve Sivadasan
human	WB	Oncotarget; PMID: 28947957; Authors - Paola Bagnato
human	WB	Int J Nanomedicine; Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.; Authors - Chen Li
human	WB	Cell Death Discov; FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.; Authors - Xinyan Jiao
human	WB	Cancer Med; MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.; Authors - Nengquan Sheng
rat	WB	J Sex Med; FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.; Authors - Kai Cui

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