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Cofilin Monoclonal antibody

Cofilin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, rat, pig, rabbit







Cat no : 66057-1-Ig


18 kDa phosphoprotein, CFL, CFL1, Cofilin, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, SH-SY5Y cells, A431 cells, PC-12 cells, Neuro-2a cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66057-1-Ig targets Cofilin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cofilin fusion protein Ag18044
Full Name cofilin 1 (non-muscle)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC012318
Gene Symbol CFL1
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.


Product Specific Protocols
WB protocol for Cofilin antibody 66057-1-IgDownload protocol
IHC protocol for Cofilin antibody 66057-1-IgDownload protocol
IF protocol for Cofilin antibody 66057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization

Authors - Weiji Weng

Mol Psychiatry

Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.

Authors - Yukie Yamahashi

Nat Commun

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

Authors - Sergi Marco

Nat Commun

Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.

Authors - Emmanouela Kallergi


Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A

Authors - Blake A Creighton

Int J Nanomedicine

Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.

Authors - Chen Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Marina (Verified Customer) (03-12-2024)

Samples are two tumour (glioblastoma) cell lines (TC1, TC2). Gradient gel 4-15 %. Dilution 1:5000, primary antibody incubation 4ºC overnight, secondary antibody incubation 1 h room temperature. The bands appear at the expected size of the target protein.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Human tumour cell lines
Cofilin Antibody Western Blot validation (1:5000 dilution) in Human tumour cell lines (Cat no:66057-1-Ig)