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Cofilin Monoclonal antibody

Cofilin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G6A2

Cat no : 66057-1-Ig

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-12 cells, Neuro-2a cells, pig brain, rat brain, mouse brain, rabbit brain, SH-SY5Y cells, A431 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66057-1-Ig is Cofilin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cofilin fusion protein Ag18044
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC012318
Gene symbol CFL1
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for Cofilin antibody 66057-1-IgDownload protocol
IHC protocol for Cofilin antibody 66057-1-IgDownload protocol
IF protocol for Cofilin antibody 66057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Nat Neurosci

C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.

Authors - Rajeeve Sivadasan
humanWB

Oncotarget

PMID: 28947957

Authors - Paola Bagnato
humanWB

Int J Nanomedicine

Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.

Authors - Chen Li
humanWB

Cancer Med

MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Authors - Nengquan Sheng
ratWB

J Sex Med

FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.

Authors - Kai Cui
humanWB

Front Microbiol

Involvement of Actin-Regulating Factor Cofilin in the Inclusion Body Formation and RNA Synthesis of Human Parainfluenza Virus Type 3 via Interaction With the Nucleoprotein.

Authors - Yinshuang Li
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