Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, human brain tissue, human heart tissue, human ileum tissue, human kidney tissue, human placenta tissue, mouse brain tissue, NIH/3T3 cells|
|Positive IHC detected in||human liver cancer tissue, human liver tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, PFA fixed cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12527-1-AP targets CHMP2B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHMP2B fusion protein Ag3222|
|Full Name||chromatin modifying protein 2B|
|Calculated molecular weight||24 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC001553|
|Gene ID (NCBI)||25978|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CHMP2B, chromatin-modifying protein 2b, also named as CHMP2.5, VPS2B and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or been replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, maybe caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.
Metab Brain Dis
Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome.
J Cell Biochem
Iron regulatory protein 1 suppresses hypoxia-induced iron uptake proteins expression and decreases iron levels in HepG2 cells.
J Cardiovasc Transl Res
SIRT6 Protects Against Myocardial Ischemia-Reperfusion Injury by Attenuating Aging-Related CHMP2B Accumulation.
Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.
Int J Parasitol Drugs Drug Resist
Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.