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CHMP2B Polyclonal antibody

CHMP2B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12527-1-AP

Synonyms

CHMP2.5, CHMP2B, chromatin modifying protein 2B, DMT1, hVps2 2, VPS2 2, VPS2B



Tested Applications

Positive WB detected inSH-SY5Y cells, human brain tissue, human heart tissue, human ileum tissue, human kidney tissue, human placenta tissue, mouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, PFA fixed cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12527-1-AP targets CHMP2B in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP2B fusion protein Ag3222
Full Name chromatin modifying protein 2B
Calculated molecular weight 24 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001553
Gene symbol CHMP2B
Gene ID (NCBI) 25978
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHMP2B, chromatin-modifying protein 2b, also named as CHMP2.5, VPS2B and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or been replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, maybe caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.

Protocols

Product Specific Protocols
WB protocol for CHMP2B antibody 12527-1-APDownload protocol
IHC protocol for CHMP2B antibody 12527-1-APDownload protocol
IF protocol for CHMP2B antibody 12527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Metab Brain Dis

Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome.

Authors - Wenqi Gao
humanWB

J Cell Biochem

Iron regulatory protein 1 suppresses hypoxia-induced iron uptake proteins expression and decreases iron levels in HepG2 cells.

Authors - Chun-Ming Cheng
humanWB

J Virol

Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.

Authors - Nicholas T Streck
humanWB

Int J Parasitol Drugs Drug Resist

Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.

Authors - Cristina Ballesteros
mouseIF

Sci Rep

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Authors - Carolyn Ott
humanWB

Oncotarget

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Authors - Yi Huo