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CIRBP Polyclonal antibody
CIRBP Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 10209-2-AP
|Positive WB detected in||PC-12 cells, PC-13 cells, HEK-293 cells, HepG2 cells, A549 cells, mouse brain tissue, mouse testis tissue, Y79 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human breast cancer tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10209-2-AP targets CIRBP in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, squirrel|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CIRBP fusion protein Ag0280|
|Full Name||cold inducible RNA binding protein|
|Calculated molecular weight||172 aa, 19 kDa|
|Observed molecular weight||19 kDa|
|GenBank accession number||BC000403|
|Gene ID (NCBI)||1153|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.
|Product Specific Protocols|
|WB protocol for CIRBP antibody 10209-2-AP||Download protocol|
|IHC protocol for CIRBP antibody 10209-2-AP||Download protocol|
|IF protocol for CIRBP antibody 10209-2-AP||Download protocol|
|IP protocol for CIRBP antibody 10209-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Ther Nucleic Acids
Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia.
Therapeutic hypothermia protects photoreceptors through activating Cirbp pathway.
Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis.
Extracellular CIRP induces macrophage endotoxin tolerance through IL-6R-mediated STAT3 activation.
Front Cell Dev Biol
The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.
J Thorac Cardiovasc Surg
Cold-inducible RNA-binding protein as a novel target to alleviate blood-brain barrier damage induced by cardiopulmonary bypass.