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  • KD/KO Validated

CIRBP Polyclonal antibody

CIRBP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 10209-2-AP

Synonyms

A18 hnRNP, A18HNRNP, CIRBP, CIRP



Tested Applications

Positive WB detected inPC-12 cells, PC-13 cells, HEK-293 cells, HepG2 cells, A549 cells, mouse brain tissue, mouse testis tissue, Y79 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10209-2-AP targets CIRBP in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIRBP fusion protein Ag0280
Full Name cold inducible RNA binding protein
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC000403
Gene symbol CIRBP
Gene ID (NCBI) 1153
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

Protocols

Product Specific Protocols
WB protocol for CIRBP antibody 10209-2-APDownload protocol
IHC protocol for CIRBP antibody 10209-2-APDownload protocol
IF protocol for CIRBP antibody 10209-2-APDownload protocol
IP protocol for CIRBP antibody 10209-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia.

Authors - Angela Downie Ruiz Velasco
mouseWB

Neurochem Int

Therapeutic hypothermia protects photoreceptors through activating Cirbp pathway.

Authors - Ying-Jian Sun
  • KD Validated
mouseIF

JCI Insight

Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis.

Authors - Naomi-Liza Denning
mouseIF

JCI Insight

Extracellular CIRP induces macrophage endotoxin tolerance through IL-6R-mediated STAT3 activation.

Authors - Mian Zhou
mouseWB

Front Cell Dev Biol

The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.

Authors - Jingyi Yan
ratWB

J Thorac Cardiovasc Surg

Cold-inducible RNA-binding protein as a novel target to alleviate blood-brain barrier damage induced by cardiopulmonary bypass.

Authors - Mingyue Liu
  • KO Validated