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CIRBP Polyclonal antibody

CIRBP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10209-2-AP

Synonyms

A18 hnRNP, A18HNRNP, CIRBP, CIRP



Tested Applications

Positive WB detected inPC-12 cells, Y79 cells, HepG2 cells, A549 cells, mouse brain tissue, PC-13 cells, HEK-293 cells, mouse testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10209-2-AP targets CIRBP in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIRBP fusion protein Ag0280
Full Name cold inducible RNA binding protein
Calculated Molecular Weight 172 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC000403
Gene Symbol CIRBP
Gene ID (NCBI) 1153
RRIDAB_2080263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

Protocols

Product Specific Protocols
WB protocol for CIRBP antibody 10209-2-APDownload protocol
IHC protocol for CIRBP antibody 10209-2-APDownload protocol
IF protocol for CIRBP antibody 10209-2-APDownload protocol
IP protocol for CIRBP antibody 10209-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.

Authors - Diego Peretti
human,squirrelWB

Cell

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.

Authors - Jingxing Ou
ratWB

Nat Med

Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis.

Authors - Qiang Xiaoling X
humanWB

Mol Cell

Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

Authors - Nandan S Gokhale
humanWB

Nucleic Acids Res

Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection

Authors - Morgane Corre
humanWB

EBioMedicine

Hypothermic Preconditioning of Human Cortical Neurons Requires Proteostatic Priming.

Authors - Nina Marie Rzechorzek