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CISD2-Specific Monoclonal antibody

CISD2-Specific Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, zebrafish, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D7A3

Cat no : 66082-1-Ig

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2



Tested Applications

Positive WB detected infetal human brain tissue, MCF-7 cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, zebrafish tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66082-1-Ig is CISD2-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human, zebrafish, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name CDGSH iron sulfur domain 2
Observed molecular weight 15 kDa
GenBank accession numberNM_001008388
Gene symbol CISD2
Gene ID (NCBI) 493856
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.

Protocols

Product Specific Protocols
WB protocol for CISD2-Specific antibody 66082-1-IgDownload protocol
IHC protocol for CISD2-Specific antibody 66082-1-IgDownload protocol
IF protocol for CISD2-Specific antibody 66082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols