- Featured Product
- KD/KO Validated
CISD2-Specific Monoclonal antibody
CISD2-Specific Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66082-1-Ig
Validation Data Gallery
|Positive WB detected in
|fetal human brain tissue, MCF-7 cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, zebrafish tissue
|Positive IHC detected in
|human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IHC : 1:20-1:200
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 66082-1-Ig is CISD2-Specific Fusion Protein expressed in E. coli.
|human, zebrafish, rat, mouse
|Host / Isotype
|Mouse / IgG2b
|CDGSH iron sulfur domain 2
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.