Claudin 15 Polyclonal antibody

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  WB analysis using 16302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16302-1-AP (Claudin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Claudin 15 Polyclonal Antibody for WB, ELISA

Cat No. 16302-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CLDN15, Claudin-15

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse small intestine tissue, rat colon tissue, rat small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16302-1-AP targets Claudin 15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 15 fusion protein Ag9124
• Full Name: claudin 15
• Calculated Molecular Weight: 128aa,14 kDa; 228aa,24 kDa
• Observed Molecular Weight: 24-26 kDa
• GenBank Accession Number: BC010160
• Gene Symbol: Claudin 15
• Gene ID (NCBI): 24146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56746
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin family member Claudin-15 is a classic tight junction molecule.