CLEC14A Polyclonal antibody

CLEC14A Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 23964-1-AP

Synonyms

C14orf27, CEG1, CLEC14A



Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, human heart tissue, rat brain tissue
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23964-1-AP targets CLEC14A in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC14A fusion protein Ag21081
Full Name C-type lectin domain family 14, member A
Calculated Molecular Weight 490 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC031567
Gene Symbol CLEC14A
Gene ID (NCBI) 161198
RRIDAB_2879377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

Protocols

Product Specific Protocols
WB protocol for CLEC14A antibody 23964-1-APDownload protocol
FC protocol for CLEC14A antibody 23964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

FASEB J

CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.

Authors - Zeyu Su