CLEC2L Polyclonal antibody

• 

  WB analysis using 31928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31928-1-AP (CLEC2L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 31928-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31928-1-AP (CLEC2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 31928-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31928-1-AP (CLEC2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 31928-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31928-1-AP (CLEC2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 31928-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31928-1-AP (CLEC2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CLEC2L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31928-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C-Type Lectin Domain Family 2, Member L, C-type lectin domain family 2 member L

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse cerebellum tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31928-1-AP targets CLEC2L in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLEC2L fusion protein Ag35450
• Full Name: C-type lectin domain family 2, member L
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_001080511.2
• Gene Symbol: CLEC2L
• Gene ID (NCBI): 154790
• RRID: AB_3670148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P0C7M8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-type lectin domain family 2 member L (CLEC2L) is a membrane protein mainly expressed in brain tissue and probably has carbohydrate-binding activity. The CLEC2L-encoded C-type lectin-like receptors (CTLRs) are expressed as non-glycosylated, disulfide-linked homodimers at the cell surface, and designated as BACL (brain-associated C-type lectin) (PMID: 23776472). The CLEC2L protein has 214 amino acids with a predicted molecular mass of 23.9 kDa. Under non-reducing conditions, FLAG-tagged CLEC2L proteins appeared with a molecular mass of ~55 kDa in immunoblots because of the occurrence of disulfide-linked homodimers (PMID: 23776472).