CLPP Polyclonal antibody

CLPP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15698-1-AP

Synonyms

CLPP, Endopeptidase Clp



Tested Applications

Positive WB detected inhuman placenta tissue, rat heart tissue, K-562 cells, mouse heart tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15698-1-AP targets CLPP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLPP fusion protein Ag8325
Full Name ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Calculated molecular weight 30 kDa
Observed molecular weight 26 kDa, 30 kDa
GenBank accession numberBC002956
Gene symbol CLPP
Gene ID (NCBI) 8192
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Protocols

Product Specific Protocols
WB protocol for CLPP antibody 15698-1-APDownload protocol
IHC protocol for CLPP antibody 15698-1-APDownload protocol
IF protocol for CLPP antibody 15698-1-APDownload protocol
IP protocol for CLPP antibody 15698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nucleic Acids Res

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Authors - Danni Chen
humanWB

PLoS Genet

TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.

Authors - Peng Wang
humanWB

Genes Genomics

SIRT3-mediated mitochondrial unfolded protein response weakens breast cancer sensitivity to cisplatin.

Authors - Hao Chen
ratWB

Bioengineered

Mitochondrial serine protease Omi/HtrA2 accentuates brain ischemia/reperfusion injury in rats and oxidative stress injury in vitro by modulating mitochondrial stress proteins CHOP and ClpP and physically interacting with mitochondrial fusion protein OPA1.

Authors - Hailong You
mouseWB

Neurogenetics

Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.

Authors - Antonia Maletzko
humanWB

Front Genet

Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population.

Authors - Dayan Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anh (Verified Customer) (07-15-2021)

very good, very clear bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: heart cells
FH

Mariusz (Verified Customer) (02-06-2019)

Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:2000 (Western), 1:200 (IF)
  • Cell Tissue Type: HeLa and HCt116