|Positive WB detected in||human placenta tissue, rat heart tissue, K-562 cells, mouse heart tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue, human testis tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15698-1-AP targets CLPP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CLPP fusion protein Ag8325|
|Full Name||ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)|
|Calculated molecular weight||30 kDa|
|Observed molecular weight||26 kDa, 30 kDa|
|GenBank accession number||BC002956|
|Gene ID (NCBI)||8192|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.
|Product Specific Protocols|
|WB protocol for CLPP antibody 15698-1-AP||Download protocol|
|IHC protocol for CLPP antibody 15698-1-AP||Download protocol|
|IF protocol for CLPP antibody 15698-1-AP||Download protocol|
|IP protocol for CLPP antibody 15698-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mariusz (Verified Customer) (02-06-2019)
Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.