CLPP Polyclonal antibody
CLPP Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15698-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, rat heart tissue, K-562 cells, mouse heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue, human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
Product Information
15698-1-AP targets CLPP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CLPP fusion protein Ag8325 |
| Full Name | ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli) |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 26 kDa, 30 kDa |
| GenBank accession number | BC002956 |
| Gene symbol | CLPP |
| Gene ID (NCBI) | 8192 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLPP antibody 15698-1-AP | Download protocol |
| IHC protocol for CLPP antibody 15698-1-AP | Download protocol |
| IF protocol for CLPP antibody 15698-1-AP | Download protocol |
| IP protocol for CLPP antibody 15698-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism. | ||
PLoS Genet TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response. | ||
PLoS One The transcription factor C/EBP delta has anti-apoptotic and anti-inflammatory roles in pancreatic beta cells. | ||
Mol Cell Biol LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response. | ||
Int J Biol Sci Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1G93A Mice. | ||
J Biol Chem Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Mariusz (Verified Customer) (02-06-2019) | Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.
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