|Positive WB detected in||human placenta tissue, rat heart tissue, K-562 cells, mouse heart tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue, human testis tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15698-1-AP targets CLPP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CLPP fusion protein Ag8325|
|Full Name||ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)|
|Calculated molecular weight||30 kDa|
|Observed molecular weight||26 kDa, 30 kDa|
|GenBank accession number||BC002956|
|Gene ID (NCBI)||8192|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.
|Product Specific Protocols|
|WB protocol for CLPP antibody 15698-1-AP||Download protocol|
|IHC protocol for CLPP antibody 15698-1-AP||Download protocol|
|IF protocol for CLPP antibody 15698-1-AP||Download protocol|
|IP protocol for CLPP antibody 15698-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nucleic Acids Res
Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.
TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.
SIRT3-mediated mitochondrial unfolded protein response weakens breast cancer sensitivity to cisplatin.
Mitochondrial serine protease Omi/HtrA2 accentuates brain ischemia/reperfusion injury in rats and oxidative stress injury in vitro by modulating mitochondrial stress proteins CHOP and ClpP and physically interacting with mitochondrial fusion protein OPA1.
Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.
Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anh (Verified Customer) (07-15-2021)
very good, very clear bands
Mariusz (Verified Customer) (02-06-2019)
Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.