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CLPP Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 15698-1-AP

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

CLPP Antibody WB human placenta tissue 15698-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

CLPP Antibody IF Hela cells 15698-1-AP

Immunofluorescent analysis of Hela cells, using CLPP antibody 15698-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CLPP Antibody IP mouse skeletal muscle tissue 15698-1-AP

IP Result of anti-CLPP (IP:15698-1-AP, 3ug; Detection:15698-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

CLPP Antibody IHC human liver cancer tissue 15698-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15698-1-AP( CLPP Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CLPP Antibody IHC human liver cancer tissue 15698-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15698-1-AP( CLPP Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
human placenta tissue, rat heart tissue, K-562 cells, mouse heart tissue
Positive IP detected in:
mouse skeletal muscle tissue
Positive IHC detected in:
human liver cancer tissue, human pancreas tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human liver tissue, human spleen tissue, human lung tissue, human ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CLPP fusion protein Ag8325
Full name:
ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Calculated molecular weight:
30 kDa
Observed molecular weight:
26 kDa; 30 kDa
GenBank accession number:
BC002956
Gene ID (NCBI):
8192
Gene symbol
CLPP
Synonyms
CLPP, Endopeptidase Clp

Background

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

CLPP Rabbit Polyclonal antibody

Mariusz (Verified Customer), 02-06-2019
Applications
Western Blot,Immunofluorescence,
Primary Antibody Dilution
1:2000 (Western), 1:200 (IF)
No image submitted with review

No image submitted with review

Cell/Tissue Type
HeLa and HCt116
Lot Number
Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.

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