CLPP Polyclonal antibody

CLPP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (2)

Cat no : 15698-1-AP

Synonyms

CLPP, Endopeptidase Clp

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Validation Data Gallery

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  WB analysis using 15698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15698-1-AP

  Immunofluorescent analysis of Hela cells, using CLPP antibody 15698-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IP experiment of mouse skeletal muscle using 15698-1-AP

  IP Result of anti-CLPP (IP:15698-1-AP, 3ug; Detection:15698-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, rat heart tissue, K-562 cells, mouse heart tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human liver cancer tissue, human kidney tissue, human testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 24 publications below
• IF: See 1 publications below

Product Information

15698-1-AP targets CLPP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLPP fusion protein Ag8325
• Full Name: ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
• Calculated molecular weight: 30 kDa
• Observed molecular weight: 26 kDa, 30 kDa
• GenBank accession number: BC002956
• Gene symbol: CLPP
• Gene ID (NCBI): 8192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Publications

Species	Application	Title
zebrafish	WB	Nucleic Acids Res; Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.; Authors - Danni Chen
human	WB	PLoS Genet; TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.; Authors - Peng Wang
human	WB	Genes Genomics; SIRT3-mediated mitochondrial unfolded protein response weakens breast cancer sensitivity to cisplatin.; Authors - Hao Chen
rat	WB	Bioengineered; Mitochondrial serine protease Omi/HtrA2 accentuates brain ischemia/reperfusion injury in rats and oxidative stress injury in vitro by modulating mitochondrial stress proteins CHOP and ClpP and physically interacting with mitochondrial fusion protein OPA1.; Authors - Hailong You
mouse	WB	Neurogenetics; Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.; Authors - Antonia Maletzko
human	WB	Front Genet; Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population.; Authors - Dayan Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anh (Verified Customer) (07-15-2021)

very good, very clear bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart cells

FH

Mariusz (Verified Customer) (02-06-2019)

Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.

• Applications: Western Blot,Immunofluorescence,
• Primary Antibody Dilution: 1:2000 (Western), 1:200 (IF)
• Cell Tissue Type: HeLa and HCt116