COG4 Polyclonal antibody, PBS Only

• 

  WB analysis using 31539-1-AP (same clone as 31539-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31539-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 31539-1-AP (same clone as 31539-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31539-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 31539-1-AP (same clone as 31539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31539-1-AP (COG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31539-1-PBS in a different storage buffer formulation.

COG4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 31539-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

COD1, COG complex subunit 4

---

Product Information

31539-1-PBS targets COG4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35295

  Product name: Recombinant human COG4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 506-785 aa of NM_001365426.1

  Sequence: FQDIQRGVTSAVNIMHSSLQQGKFDTKGIESTDEAKMSFLVTLNNVEVCSENISTLKKTLESDCTKLFSQGIGGEQAQAKFDSCLSDLAAVSNKFRDLLQEGLTELNSTAIKPQVQPWINSFFSVSHNIEEEEFNDYEANDPWVQQFILNLEQQMAEFKASLSPVIYDSLTGLMTSLVAVELEKVVLKSTFNRLGGLQFDKELRSLIAYLTTVTTWTIRDKFARLSQMATILNLERVTEILDYWGPNSGPLTWRLTPAEVRQVLALRIDFRSEDIKRLRL

• Full Name: component of oligomeric golgi complex 4
• Calculated Molecular Weight: 89kDa，785aa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: NM_001365426.1
• Gene Symbol: COG4
• Gene ID (NCBI): 25839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H9E3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

COG complex subunit 4 is present in high molecular weight complexes that govern membrane movement between the Golgi and ER. Cog4 dependent trafficking and secretion of ECM components are required for protrusion and extension of the epithelial projections of the inner ear (PMID: 30287385). Loss-of-function COG4-mutant cells have impaired glycosylation and reduced COG4 protein levels (PMID: 30290151).