Coilin Polyclonal antibody

Coilin Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG







Cat no : 10967-1-AP


CLN80, COIL, Coilin, p80, p80 coilin

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HEK-293 cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293T cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10967-1-AP targets Coilin in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Coilin fusion protein Ag1416
Full Name coilin
Calculated molecular weight 62 aa, 6 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC010385
Gene symbol COIL
Gene ID (NCBI) 8161
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs


Product Specific Protocols
WB protocol for Coilin antibody 10967-1-APDownload protocol
IHC protocol for Coilin antibody 10967-1-APDownload protocol
IF protocol for Coilin antibody 10967-1-APDownload protocol
IP protocol for Coilin antibody 10967-1-APDownload protocol
FC protocol for Coilin antibody 10967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Exp Cell Res

EphB4 localises to the nucleus of prostate Cancer cells.

Authors - Inga Mertens-Walker

PLoS One

FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.

Authors - Chen Song S

PLoS One

Protein arginine methyltransferase 5 functions in opposite ways in the cytoplasm and nucleus of prostate cancer cells.

Authors - Gu Zhongping Z

J Biol Chem

Identification of Small Molecule Inhibitors of Pre-mRNA Splicing.

Authors - Andrea Pawellek

J Biol Chem

Identification of small molecule inhibitors of pre-mRNA splicing.

Authors - Andrea Pawallek

Neurobiol Dis

UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.

Authors - Simona Rossi


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human satelllite muscle cells
Coilin Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:10967-1-AP)