|Positive WB detected in||Jurkat cells, K-562 cells, HEK-293 cells, human brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human prostate cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10967-1-AP targets Coilin in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Coilin fusion protein Ag1416|
|Calculated molecular weight||62 aa, 6 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC010385|
|Gene ID (NCBI)||8161|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs
|Product Specific Protocols|
|WB protocol for Coilin antibody 10967-1-AP||Download protocol|
|IHC protocol for Coilin antibody 10967-1-AP||Download protocol|
|IF protocol for Coilin antibody 10967-1-AP||Download protocol|
|IP protocol for Coilin antibody 10967-1-AP||Download protocol|
|FC protocol for Coilin antibody 10967-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Exp Cell Res
EphB4 localises to the nucleus of prostate Cancer cells.
FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.
Protein arginine methyltransferase 5 functions in opposite ways in the cytoplasm and nucleus of prostate cancer cells.
J Biol Chem
Identification of Small Molecule Inhibitors of Pre-mRNA Splicing.
J Biol Chem
Identification of small molecule inhibitors of pre-mRNA splicing.
UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Baptiste (Verified Customer) (09-05-2019)
This antibody works well in western blot