Coilin Antibody

Coilin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10967-1-AP

Synonyms

CLN80, COIL, Coilin, p80, p80 coilin



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HEK-293 cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10967-1-AP targets Coilin in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Coilin fusion protein Ag1416
Full Name coilin
Calculated molecular weight 62.6 kDa
Observed molecular weight 80 kDa
GenBank accession number BC010385
Gene symbol COIL
Gene ID (NCBI) 8161
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

Protocols

Product Specific Protocols
WB protocol for Coilin antibody 10967-1-APDownload protocol
IHC protocol for Coilin antibody 10967-1-APDownload protocol
IF protocol for Coilin antibody 10967-1-APDownload protocol
IP protocol for Coilin antibody 10967-1-APDownload protocol
FC protocol for Coilin antibody 10967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Exp Cell Res

EphB4 localises to the nucleus of prostate Cancer cells.

Authors - Inga Mertens-Walker
humanWB

PLoS One

FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.

Authors - Chen Song S
humanIF

PLoS One

Protein arginine methyltransferase 5 functions in opposite ways in the cytoplasm and nucleus of prostate cancer cells.

Authors - Gu Zhongping Z
humanIF

J Biol Chem

Identification of small molecule inhibitors of pre-mRNA splicing.

Authors - Andrea Pawallek
humanIF

J Biol Chem

Identification of Small Molecule Inhibitors of Pre-mRNA Splicing.

Authors - Andrea Pawellek
humanWB,IF

Neurobiol Dis

UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.

Authors - Simona Rossi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Baptiste Bogard (09-05-2019)

This antibody works well in western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human satelllite muscle cells
Coilin Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:10967-1-AP)