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Coilin Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 10967-1-AP

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

Coilin Antibody WB human brain tissue 10967-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 10967-1-AP(Coilin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Coilin Antibody IHC human prostate cancer tissue 10967-1-AP

Immunohistochemical of paraffin-embedded human prostate cancer using 10967-1-AP(Coilin antibody) at dilution of 1:100 (under 10x lens)

Coilin Antibody IP HEK-293 cells 10967-1-AP

IP Result of anti-Coilin (IP:10967-1-AP, 3ug; Detection:10967-1-AP 1:500) with HEK-293 cells lysate 1000ug.

Coilin Antibody IF HepG2 cells 10967-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 10967-1-AP(Coilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Coilin Antibody FC HEK-293T cells 10967-1-AP

1X10^6 HEK-293T cells were stained with 0.2ug Coilin antibody (10967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
human brain tissue, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human prostate cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HEK-293T cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Coilin fusion protein Ag1416
Full name:
coilin
Calculated molecular weight:
62.6 kDa
Observed molecular weight:
80 kDa
GenBank accession number:
BC010385
Gene ID (NCBI):
8161
Gene symbol
COIL
Synonyms
CLN80, COIL, Coilin, p80, p80 coilin

Background

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs


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