Coilin Polyclonal antibody

Coilin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10967-1-AP

Synonyms

CLN80, COIL, Coilin, p80, p80 coilin

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Validation Data Gallery

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  WB analysis of Jurkat using 10967-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10967-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10967-1-AP (Coilin antibody) at dilution of 1:100 (under 10x lens).

• 

  IP experiment of HEK-293 using 10967-1-AP

  IP Result of anti-Coilin (IP:10967-1-AP, 3ug; Detection:10967-1-AP 1:500) with HEK-293 cells lysate 1000ug.

• 

  IF Staining of HepG2 using 10967-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10967-1-AP (Coilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293T using 10967-1-AP

  1X10^6 HEK-293T cells were stained with 0.2ug Coilin antibody (10967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, HEK-293 cells, human brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 7 publications below

Product Information

10967-1-AP targets Coilin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Coilin fusion protein Ag1416
• Full Name: coilin
• Calculated molecular weight: 62 aa, 6 kDa
• Observed molecular weight: 80 kDa
• GenBank accession number: BC010385
• Gene symbol: COIL
• Gene ID (NCBI): 8161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

Publications

Species	Application	Title
human	IF	Exp Cell Res; EphB4 localises to the nucleus of prostate Cancer cells.; Authors - Inga Mertens-Walker
human	WB	PLoS One; FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.; Authors - Chen Song S
human	IF	PLoS One; Protein arginine methyltransferase 5 functions in opposite ways in the cytoplasm and nucleus of prostate cancer cells.; Authors - Gu Zhongping Z
human	IF	J Biol Chem; Identification of Small Molecule Inhibitors of Pre-mRNA Splicing.; Authors - Andrea Pawellek
human	IF	J Biol Chem; Identification of small molecule inhibitors of pre-mRNA splicing.; Authors - Andrea Pawallek
human	WB,IF	Neurobiol Dis; UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.; Authors - Simona Rossi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human satelllite muscle cells

Coilin Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:10967-1-AP)