Collagen Type I Monoclonal antibody

Collagen Type I Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1


Human, pig, mouse, rat and More (3)







Cat no : 66761-1-Ig


Alpha 2 type I collagen, COL1A2, Collagen alpha 2(I) chain, Collagen Type I, collagen, type I, alpha 2, OI4

Tested Applications

Positive WB detected inrat skin tissue, pig skin tissue, mouse tail tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66761-1-Ig targets Collagen Type I in WB, IF, IHC, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Reactivity Human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281
Full Name collagen, type I, alpha 2
Calculated Molecular Weight 1366 aa, 130 kDa
Observed Molecular Weight 140 kDa, 70-80 kDa
GenBank Accession NumberBC054498
Gene Symbol COL1A2
Gene ID (NCBI) 1278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.


Product Specific Protocols
WB protocol for Collagen Type I antibody 66761-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Su (Verified Customer) (03-18-2022)

Works good and consistent.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Cancer tissue
Collagen Type I Antibody Immunohistochemistry validation (1:250 dilution) in Cancer tissue (Cat no:66761-1-Ig)