|Positive WB detected in||rat skin tissue, pig skin tissue, mouse tail tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Sample-dependent, check data in validation data gallery|
66761-1-Ig targets Collagen Type I in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.
|Tested Reactivity||Human, pig, mouse, rat|
|Cited Reactivity||human, mouse, rat, rabbit, bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Collagen Type I fusion protein Ag6281|
|Full Name||collagen, type I, alpha 2|
|Calculated molecular weight||1366 aa, 130 kDa|
|Observed molecular weight||140 kDa, 70-80 kDa|
|GenBank accession number||BC054498|
|Gene ID (NCBI)||1278|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Su (Verified Customer) (03-18-2022)
Works good and consistent.