Collagen Type I Monoclonal antibody

Collagen Type I Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D5E8

Cat no : 66761-1-Ig

Synonyms

Alpha 2 type I collagen, COL1A2, Collagen alpha 2(I) chain, Collagen Type I, collagen, type I, alpha 2, OI4



Tested Applications

Positive WB detected inrat skin tissue, pig skin tissue, mouse tail tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66761-1-Ig targets Collagen Type I in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Reactivity Human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281
Full Name collagen, type I, alpha 2
Calculated molecular weight 1366 aa, 130 kDa
Observed molecular weight 140 kDa, 70-80 kDa
GenBank accession numberBC054498
Gene symbol COL1A2
Gene ID (NCBI) 1278
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.

Protocols

Product Specific Protocols
WB protocol for Collagen Type I antibody 66761-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Mol Med

Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.

Authors - Liang-Liang Fan
ratWB

Front Cell Dev Biol

Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat

Authors - Huangrong Zhu
mouseWB,IHC

Int Immunopharmacol

Zanubrutinib attenuates bleomycin-induced pulmonary fibrosis by inhibiting the TGF-β1 signaling pathway

Authors - Shanshan Chen
humanWB

Int Immunopharmacol

Amygdalin inhibits TGFβ1-induced activation of hepatic stellate cells (HSCs) in vitro and CCl4-induced hepatic fibrosis in rats in vivo.

Authors - Ruoyu Wang
WB,IHC

Front Bioeng Biotechnol

Primary Repair for Treating Acute Proximal Anterior Cruciate Ligament Tears: A Histological Analysis and Prospective Clinical Trial.

Authors - Yue Yang
mouseWB

Toxicol Lett

A lncRNA Gpr137b-ps/miR-200a-3p/CXCL14 axis modulates hepatic stellate cell (HSC) activation.

Authors - Jinmao Liao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Su (Verified Customer) (03-18-2022)

Works good and consistent.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Cancer tissue
Collagen Type I Antibody Immunohistochemistry validation (1:250 dilution) in Cancer tissue (Cat no:66761-1-Ig)