CP110 Polyclonal antibody

• Featured Product
• KD/KO Validated

CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (2)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

86

Reviews (2)

Cat no : 12780-1-AP

Synonyms

Centrosomal protein of 110 kDa, CP110, CP110 protein, KIAA0419

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Validation Data Gallery

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  WB analysis using 12780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12780-1-AP (CP110 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MEF/HeLa cells using 12780-1-AP

  IF result (Centrioles) of anti-CP110 (12780-1-AP) by Dr. Corbit Kevin.

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  WB analysis of HeLa cells using 12780-1-AP

  WB result of CP110 antibody (12780-1-AP, 1:500) with si-control and si-CP110 transfected HeLa cells.

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  IP experiment of HeLa using 12780-1-AP

  IP Result of anti-CP110 (IP:12780-1-AP, 3ug; Detection:12780-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human testis using 12780-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12780-1-AP (CP110 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 12780-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12780-1-AP (CP110 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HeLa using 12780-1-AP

  1X10^6 HeLa cells were stained with .2ug CP110 antibody (12780-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MEF/HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 70 publications below
• IP: See 1 publications below

Product Information

12780-1-AP targets CP110 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: Drosophila, human, mouse, rat, Xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CP110 fusion protein Ag3489
• Full Name: CP110 protein
• Calculated molecular weight: 991 aa, 111 kDa
• Observed molecular weight: 113 kDa
• GenBank accession number: BC036654
• Gene symbol: CP110
• Gene ID (NCBI): 9738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

Publications

Species	Application	Title
		Mol Biol Cell; PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion.; Authors - Chii Shyang Fong
human	IF	Nat Commun; Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.; Authors - Christine Insinna
human	IF	Nat Commun; The Cep57-pericentrin module organizes PCM expansion and centriole engagement.; Authors - Koki Watanabe
human	IF	Protein Cell; Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.; Authors - Hongmei Mao
human	IF	Nat Commun; Self-organization of Plk4 regulates symmetry breaking in centriole duplication.; Authors - Shohei Yamamoto
human	IF	Sci Rep; CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis.; Authors - Jhih-Jie Tsai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (02-16-2021)

Works very well for detection of CP110 protein using western blotting and immunfluorescence

• Applications: Western Blot,Immunofluorescence
• Primary Antibody Dilution: 1:500 (western), 1:300 (IF)
• Cell Tissue Type: Jurkat E6.1 and HEK 293T

CP110 Antibody Western Blot,Immunofluorescence validation (1:500 (western), 1:300 (IF) dilution) in Jurkat E6.1 and HEK 293T (Cat no:12780-1-AP)

FH

EK (Verified Customer) (05-04-2020)

works well for immunofluorescence detection of basal body localization

• Applications: Immunofluorescence

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