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- KD/KO Validated
CP110 Polyclonal antibody
CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine and More (2)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 12780-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human testis tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MEF/HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 21 publications below |
IHC | See 1 publications below |
IF | See 70 publications below |
IP | See 1 publications below |
Product Information
12780-1-AP targets CP110 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | Drosophila, human, mouse, rat, Xenopus |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CP110 fusion protein Ag3489 |
Full Name | CP110 protein |
Calculated molecular weight | 991 aa, 111 kDa |
Observed molecular weight | 113 kDa |
GenBank accession number | BC036654 |
Gene symbol | CP110 |
Gene ID (NCBI) | 9738 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CP110 antibody 12780-1-AP | Download protocol |
IHC protocol for CP110 antibody 12780-1-AP | Download protocol |
IP protocol for CP110 antibody 12780-1-AP | Download protocol |
FC protocol for CP110 antibody 12780-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Biol Cell PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. | ||
Nat Commun Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. | ||
Nat Commun The Cep57-pericentrin module organizes PCM expansion and centriole engagement. | ||
Protein Cell Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1. | ||
Nat Commun Self-organization of Plk4 regulates symmetry breaking in centriole duplication. | ||
Sci Rep CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (02-16-2021) | Works very well for detection of CP110 protein using western blotting and immunfluorescence
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FH EK (Verified Customer) (05-04-2020) | works well for immunofluorescence detection of basal body localization
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