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- KD/KO Validated
CP110 Polyclonal antibody
CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, dog and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 12780-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells, multi-cells |
| Positive IHC detected in | human testis tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MEF/HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 20 publications below |
| IHC | See 1 publications below |
| IF | See 66 publications below |
| IP | See 1 publications below |
Product Information
12780-1-AP targets CP110 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, dog samples.
| Tested Reactivity | human, mouse, rat, dog |
| Cited Reactivity | human, mouse, rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CP110 fusion protein Ag3489 |
| Full Name | CP110 protein |
| Calculated molecular weight | 991 aa, 111 kDa |
| Observed molecular weight | 113 kDa |
| GenBank accession number | BC036654 |
| Gene symbol | CP110 |
| Gene ID (NCBI) | 9738 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CP110 antibody 12780-1-AP | Download protocol |
| IHC protocol for CP110 antibody 12780-1-AP | Download protocol |
| IP protocol for CP110 antibody 12780-1-AP | Download protocol |
| FC protocol for CP110 antibody 12780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Biol Cell PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. | ||
Nat Commun Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. | ||
Nat Commun The Cep57-pericentrin module organizes PCM expansion and centriole engagement. | ||
Protein Cell Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1. | ||
Nat Commun Self-organization of Plk4 regulates symmetry breaking in centriole duplication. | ||
Sci Rep CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH EK (Verified Customer) (05-04-2020) | works well for immunofluorescence detection of basal body localization
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