Tested Applications
| Positive WB detected in | HCT 116 cells, HeLa cells, HEK-293T cells, Jurkat cells, MOLT-4 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12780-1-AP targets CP110 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, canine, drosophila, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3489 Product name: Recombinant human CP110 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-337 aa of BC036654 Sequence: MEEYEKFCEKSLARIQEASLSTESFLPAQSESISLIRFHGVAILSPLLNIEKRKEMQQEKQKALDVEARKQVNRKKALLTRVQEILDNVQVRKAPNASDFDQWEMETVYSNSEVRNLNVPATFPNSFPSHTEHSTAAKLDKIAGILPLDNEDQCKTDGIDLARDSEGFNSPKQCDSSNISHVENEAFPKTSSATPQETLISDGPFSVNEQQDLPLLAEVIPDPYVMSLQNLMKKSKEYIEREQSRRSLRGSMNRIVNESHLDKEHDAVEVADCVKEKGQLTGKHCVSVIPDKPSLNKSNVLLQGASTQASSMSMPVLASFSKVDIPIRTGHPTVLES Predict reactive species |
| Full Name | CP110 protein |
| Calculated Molecular Weight | 991 aa, 111 kDa |
| Observed Molecular Weight | 113 kDa |
| GenBank Accession Number | BC036654 |
| Gene Symbol | CP110 |
| Gene ID (NCBI) | 9738 |
| RRID | AB_10638480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43303 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CP110 antibody 12780-1-AP | Download protocol |
| IF protocol for CP110 antibody 12780-1-AP | Download protocol |
| IHC protocol for CP110 antibody 12780-1-AP | Download protocol |
| IP protocol for CP110 antibody 12780-1-AP | Download protocol |
| WB protocol for CP110 antibody 12780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CP110 Polyclonal antibody (catalog number 12780-1-AP) has 189 citations. These appear in high-impact journals including Nature, Science, Cell, Nature Cell Biology, Nature Communications, Cell Research, EMBO Journal, Current Biology, Journal of Cell Biology, Developmental Cell, and eLife. The research fields are dominated by ciliogenesis and primary cilium formation, centriole/centrosome biogenesis and structure, cell division and spindle assembly, ciliopathies, and cancer biology, with applications mainly in immunofluorescence, Western blot, and immunoprecipitation.
| Species | Application | Title |
|---|---|---|
Nature USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110. | ||
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Cell Time-series reconstruction of the molecular architecture of human centriole assembly | ||
Nat Nanotechnol Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase. | ||
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. |
Reviews
What customers say
All three reviews rate this antibody 5 stars. Users report excellent performance for detecting CP110 protein in both Western blot and immunofluorescence applications. It was successfully used in RPE1, Jurkat E6.1, and HEK 293T cells, with recommended dilutions of 1:500 for WB and 1:200–1:300 for IF. One reviewer specifically highlighted its ability to clearly visualize basal body localization. Overall, customers found the antibody reliable and effective across multiple cell types and techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elisa (Verified Customer) (01-05-2023) | RPE1 cells stained for Hoechst (DNA marker, in green), CP110 (in magenta) and gtub (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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Sarah (Verified Customer) (02-16-2021) | Works very well for detection of CP110 protein using western blotting and immunfluorescence
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EK (Verified Customer) (05-04-2020) | works well for immunofluorescence detection of basal body localization
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