- Featured Product
- KD/KO Validated
CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12780-1-AP
Centrosomal protein of 110 kDa, CP110, CP110 protein, KIAA0419
Validation Data Gallery
|Positive WB detected in||HeLa cells, K-562 cells|
|Positive IP detected in||HeLa cells, multi-cells|
|Positive IHC detected in||human testis tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MEF/HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12780-1-AP targets CP110 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat, dog samples.
|Reactivity||human, mouse, rat, dog|
|Cited Species||human, mouse, rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CP110 fusion protein Ag3489|
|Full Name||CP110 protein|
|Calculated molecular weight||991aa,111 kDa|
|Observed molecular weight||113 kDa|
|GenBank accession number||BC036654|
|Gene ID (NCBI)||9738|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.
|Product Specific Protocols|
|WB protocol for CP110 antibody 12780-1-AP||Download protocol|
|IHC protocol for CP110 antibody 12780-1-AP||Download protocol|
|IP protocol for CP110 antibody 12780-1-AP||Download protocol|
|FC protocol for CP110 antibody 12780-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Biol Cell
PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion.
Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.
The Cep57-pericentrin module organizes PCM expansion and centriole engagement.
Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.
Self-organization of Plk4 regulates symmetry breaking in centriole duplication.
CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
EK V (05-04-2020)
works well for immunofluorescence detection of basal body localization