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  • KD/KO Validated

CP110 Polyclonal antibody

CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (4)

Applications

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 12780-1-AP

Synonyms

Centrosomal protein of 110 kDa, CP110, CP110 protein, KIAA0419



Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293T cells, Jurkat cells, MOLT-4 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12780-1-AP targets CP110 in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, zebrafish, Drosophila, Xenopus, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CP110 fusion protein Ag3489
Full Name CP110 protein
Calculated molecular weight 991 aa, 111 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC036654
Gene symbol CP110
Gene ID (NCBI) 9738
RRIDAB_10638480
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

Protocols

Product Specific Protocols
WB protocol for CP110 antibody 12780-1-APDownload protocol
IHC protocol for CP110 antibody 12780-1-APDownload protocol
IF protocol for CP110 antibody 12780-1-APDownload protocol
IP protocol for CP110 antibody 12780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
humanIF

Nature

USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.

Authors - Li Ji J
humanIF

Nat Nanotechnol

Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase.

Authors - Ximing Shao
humanWB,IF

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
  • KD Validated
humanWB,IF

Nat Cell Biol

CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.

Authors - Wang Won-Jing WJ
mouseWB

Nat Cell Biol

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Authors - Huijie Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elisa (Verified Customer) (01-05-2023)

RPE1 cells stained for Hoechst (DNA marker, in green), CP110 (in magenta) and gtub (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)
CP110 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:12780-1-AP)
FH

Sarah (Verified Customer) (02-16-2021)

Works very well for detection of CP110 protein using western blotting and immunfluorescence

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500 (western), 1:300 (IF)
  • Cell Tissue Type: Jurkat E6.1 and HEK 293T
CP110 Antibody Western Blot,Immunofluorescence validation (1:500 (western), 1:300 (IF) dilution) in Jurkat E6.1 and HEK 293T (Cat no:12780-1-AP)
FH

EK (Verified Customer) (05-04-2020)

works well for immunofluorescence detection of basal body localization

  • Applications: Immunofluorescence