CP110 Antibody

• Featured Product
• KD/KO Validated

CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, dog and More (1)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

75

Reviews (1)

Cat no : 12780-1-AP

Synonyms

Centrosomal protein of 110 kDa, CP110, CP110 protein, KIAA0419

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Validation Data Gallery

• 

  WB analysis of HeLa cells using 12780-1-AP

  WB result of CP110 antibody (12780-1-AP, 1:500) with si-control and si-CP110 transfected HeLa cells.

• 

  IP experiment of multi-cells using 12780-1-AP

  IP result of CP110(12780-1-AP) by Dr. Corbit, Kevin.

• 

  IF staining of MEF/HeLa cells using 12780-1-AP

  IF result(Centrioles) of anti-CP110(12780-1-AP) by Dr. Corbit Kevin.

• 

  WB analysis of HeLa cells using 12780-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12780-1-AP(CP110 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

• 

  IP experiment of HeLa cells using 12780-1-AP

  IP Result of anti-CP110 (IP:12780-1-AP, 3ug; Detection:12780-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human testis tissue using 12780-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12780-1-AP( CP110 Antibody) at dilution of 1:200 (under 10x lens)

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  IHC staining of human testis tissue using 12780-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12780-1-AP( CP110 Antibody) at dilution of 1:200 (under 40x lens)

• 

  FC experiment of HeLa cells using 12780-1-AP

  1X10^6 HeLa cells were stained with .2ug CP110 antibody (12780-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: HeLa cells, multi-cells
• Positive IHC detected in: human testis tissue, human prostate cancer tissue; ; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MEF/HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 60 publications below
• IP: See 1 publications below

Product Information

12780-1-AP targets CP110 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat, dog samples.

• Reactivity: human, mouse, rat, dog
• Cited Species: human, mouse, rat, Xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CP110 fusion protein Ag3489
• Full Name: CP110 protein
• Calculated molecular weight: 991aa,111 kDa
• Observed molecular weight: 113 kDa
• GenBank accession number: BC036654
• Gene symbol: CP110
• Gene ID (NCBI): 9738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Background Information

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

Publications

Species	Application	Title
		Mol Biol Cell; PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion.; Authors - Chii Shyang Fong
human	IF	Nat Commun; Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.; Authors - Christine Insinna
human	IF	Nat Commun; The Cep57-pericentrin module organizes PCM expansion and centriole engagement.; Authors - Koki Watanabe
human	IF	Protein Cell; Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.; Authors - Hongmei Mao
human	IF	Nat Commun; Self-organization of Plk4 regulates symmetry breaking in centriole duplication.; Authors - Shohei Yamamoto
human	IF	Sci Rep; CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis.; Authors - Jhih-Jie Tsai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

EK V (05-04-2020)

works well for immunofluorescence detection of basal body localization

• Applications: Immunofluorescence

No image submitted with review

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