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CP110 Antibody 54 Publications

Rabbit Polyclonal| Catalog number: 12780-1-AP

Featured Product KD/KO validated

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

CP110 Antibody WB HeLa cells 12780-1-AP

WB result of CP110 antibody (12780-1-AP, 1:500) with si-control and si-CP110 transfected HeLa cells.

CP110 Antibody IP multi-cells 12780-1-AP

IP result of CP110(12780-1-AP) by Dr. Corbit, Kevin.

CP110 Antibody IF MEF/HeLa cells 12780-1-AP

IF result(Centrioles) of anti-CP110(12780-1-AP) by Dr. Corbit Kevin.

CP110 Antibody WB HeLa cells 12780-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 12780-1-AP(CP110 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

CP110 Antibody IP HeLa cells 12780-1-AP

IP Result of anti-CP110 (IP:12780-1-AP, 3ug; Detection:12780-1-AP 1:1000) with HeLa cells lysate 3000ug.

CP110 Antibody IHC human testis tissue 12780-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 12780-1-AP( CP110 Antibody) at dilution of 1:200 (under 10x lens)

CP110 Antibody IHC human testis tissue 12780-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 12780-1-AP( CP110 Antibody) at dilution of 1:200 (under 40x lens)

CP110 Antibody FC HeLa cells 12780-1-AP

1X10^6 HeLa cells were stained with .2ug CP110 antibody (12780-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, dog, Xenopus
Positive WB detected in:
HeLa cells, K-562 cells
Positive IP detected in:
HeLa cells, multi-cells
Positive IHC detected in:
human testis tissue, human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MEF/HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CP110 fusion protein Ag3489
Full name:
CP110 protein
Calculated molecular weight:
991aa,111 kDa
Observed molecular weight:
113 kDa
GenBank accession number:
BC036654
Gene ID (NCBI):
9738
Gene symbol
CP110
Synonyms
Centrosomal protein of 110 kDa, CP110, CP110 protein, KIAA0419

Background

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.


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