CP110 Polyclonal antibody

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  WB analysis using 12780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12780-1-AP (CP110 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 12780-1-AP

  WB result of CP110 antibody (12780-1-AP, 1:500) with si-control and si-CP110 transfected HeLa cells.

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  IP experiment of Jurkat using 12780-1-AP

  IP result of anti-CP110 (IP:12780-1-AP, 4ug; Detection:12780-1-AP 1:1500) with Jurkat cells lysate 2400 ug.

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  IHC staining of human prostate cancer using 12780-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12780-1-AP (CP110 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12780-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12780-1-AP (CP110 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 12780-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CP110 antibody (12780-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 12780-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CP110 antibody (12780-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  FC experiment of HeLa using 12780-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CP110 (12780-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

CP110 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12780-1-AP

Publications(157)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

CCP110, Centriolar coiled-coil protein of 110 kDa, Cep110, CP 110, CP110 protein

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, HEK-293T cells, Jurkat cells, MOLT-4 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells, U2OS cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 42 publications below
• IHC: See 1 publications below
• IF: See 125 publications below
• IP: See 6 publications below

Product Information

12780-1-AP targets CP110 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, canine, zebrafish, drosophila, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CP110 fusion protein Ag3489
• Full Name: CP110 protein
• Calculated Molecular Weight: 991 aa, 111 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC036654
• Gene Symbol: CP110
• Gene ID (NCBI): 9738
• RRID: AB_10638480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43303
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

Publications

Species	Application	Title
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
human	IF	Nature; USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.; Authors - Li Ji J
human	IF	Nat Nanotechnol; Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase.; Authors - Ximing Shao
human	WB,IF	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J ; KD Validated
human	WB,IF	Nat Cell Biol; CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.; Authors - Wang Won-Jing WJ
mouse	WB	Nat Cell Biol; The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.; Authors - Huijie Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (01-05-2023)

RPE1 cells stained for Hoechst (DNA marker, in green), CP110 (in magenta) and gtub (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

CP110 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:12780-1-AP)

FH

Sarah (Verified Customer) (02-16-2021)

Works very well for detection of CP110 protein using western blotting and immunfluorescence

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500 (western), 1:300 (IF)
• Cell Tissue Type: Jurkat E6.1 and HEK 293T

CP110 Antibody Western Blot,Immunofluorescence validation (1:500 (western), 1:300 (IF) dilution) in Jurkat E6.1 and HEK 293T (Cat no:12780-1-AP)

FH

EK (Verified Customer) (05-04-2020)

works well for immunofluorescence detection of basal body localization

• Applications: Immunofluorescence