CPLX2 Polyclonal antibody

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  WB analysis of mouse brain using 18149-1-AP

  mouse and rat brain tissue were subjected to SDS PAGE followed by western blot with 18149-1-AP (CPLX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18149-1-AP

  WB result of CPLX2 antibody (18149-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX2 transfected A549 cells.

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  IP experiment of A549 using 18149-1-AP

  IP result of anti-CPLX2 (IP:18149-1-AP, 4ug; Detection:18149-1-AP 1:1000) with A549 cells lysate 600 ug.

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  IHC staining of human brain using 18149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 18149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 18149-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CPLX2 antibody (18149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

CPLX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18149-1-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CPX II, CPX 2, CPLX 2, Complexin-2, 921L

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, rat brain tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

18149-1-AP targets CPLX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPLX2 fusion protein Ag12838
• Full Name: complexin 2
• Calculated Molecular Weight: 134 aa, 15 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC093706
• Gene Symbol: CPLX2
• Gene ID (NCBI): 10814
• RRID: AB_2276580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6PUV4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

Publications

Species	Application	Title
mouse	WB	Life Sci; Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction; Authors - Yu Ma
mouse	WB	Free Radic Biol Med; NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.; Authors - Wang Ziying Z ; KD Validated
mouse	WB	Behav Brain Res; Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running.; Authors - Marius Walus
human	IHC	Cancer Biomark; Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors.; Authors - Komatsu Hiroaki H
mouse	IF	bioRxiv; Role of Complexin 2 in the regulation of hormone secretion from the islet of Langerhans; Authors - Xue Wen Ng ; KD Validated
mouse	WB	Neural Regen Res; Nicotinamide adenine dinucleotide treatment confers resistance to neonatal ischemia and hypoxia: effects on neurobehavioral phenotypes; Authors - Xiaowen Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

julie (Verified Customer) (03-28-2022)

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• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: N2a cells, mouse tissue (spinal cord)