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CPLX2 Polyclonal antibody

CPLX2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18149-1-AP

Synonyms

921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18149-1-AP targets CPLX2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPLX2 fusion protein Ag12838
Full Name complexin 2
Calculated molecular weight 134 aa, 15 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC093706
Gene symbol CPLX2
Gene ID (NCBI) 10814
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

Protocols

Product Specific Protocols
WB protocol for CPLX2 antibody 18149-1-APDownload protocol
IHC protocol for CPLX2 antibody 18149-1-APDownload protocol
IF protocol for CPLX2 antibody 18149-1-APDownload protocol
IP protocol for CPLX2 antibody 18149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Brain

Absence of BBSome function leads to astrocyte reactivity in the brain.

Authors - Minati Singh
humanIHC

Cancer Biomark

Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors.

Authors - Komatsu Hiroaki H
mouseWB

Behav Brain Res

Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running.

Authors - Marius Walus
mouseWB

Free Radic Biol Med

NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.

Authors - Wang Ziying Z
  • KD Validated