Validation Data Gallery
|Positive WB detected in||mouse brain tissue, human brain tissue, rat brain tissue|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18149-1-AP targets CPLX2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CPLX2 fusion protein Ag12838|
|Full Name||complexin 2|
|Calculated molecular weight||134 aa, 15 kDa|
|Observed molecular weight||18-20 kDa|
|GenBank accession number||BC093706|
|Gene ID (NCBI)||10814|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
|Product Specific Protocols|
|WB protocol for CPLX2 antibody 18149-1-AP||Download protocol|
|IHC protocol for CPLX2 antibody 18149-1-AP||Download protocol|
|IF protocol for CPLX2 antibody 18149-1-AP||Download protocol|
|IP protocol for CPLX2 antibody 18149-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Absence of BBSome function leads to astrocyte reactivity in the brain.
Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors.
Behav Brain Res
Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running.
Free Radic Biol Med
NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.
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julie (Verified Customer) (03-28-2022)