CPLX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CPLX2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 18149-1-AP

Synonyms

921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1

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Validation Data Gallery

• 

  WB analysis of mouse brain using 18149-1-AP

  mouse and rat brain tissue were subjected to SDS PAGE followed by western blot with 18149-1-AP (CPLX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 18149-1-AP

  WB result of CPLX2 antibody (18149-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX2 transfected A549 cells.

• 

  IP experiment of A549 using 18149-1-AP

  IP result of anti-CPLX2(IP:18149-1-AP, 4ug; Detection:18149-1-AP 1:1000) with A549 cells lysate 600 ug.

• 

  IHC staining of human brain using 18149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 18149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 18149-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 18149-1-AP (CPLX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, rat brain tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

18149-1-AP targets CPLX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPLX2 fusion protein Ag12838
• Full Name: complexin 2
• Calculated molecular weight: 134 aa, 15 kDa
• Observed molecular weight: 18-20 kDa
• GenBank accession number: BC093706
• Gene symbol: CPLX2
• Gene ID (NCBI): 10814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.; Authors - Wang Ziying Z ; KD Validated
mouse	WB	Behav Brain Res; Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running.; Authors - Marius Walus
human	IHC	Cancer Biomark; Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors.; Authors - Komatsu Hiroaki H
mouse	WB	Mol Brain; Absence of BBSome function leads to astrocyte reactivity in the brain.; Authors - Minati Singh

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

julie (Verified Customer) (03-28-2022)

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• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: N2a cells, mouse tissue (spinal cord)

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