|Positive WB detected in
|mouse brain tissue, A549 cells, rat brain tissue
|Positive IP detected in
|Positive IHC detected in
|human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
18149-1-AP targets CPLX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|CPLX2 fusion protein Ag12838
|Calculated molecular weight
|134 aa, 15 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
|Product Specific Protocols
|WB protocol for CPLX2 antibody 18149-1-AP
|IHC protocol for CPLX2 antibody 18149-1-AP
|IF protocol for CPLX2 antibody 18149-1-AP
|IP protocol for CPLX2 antibody 18149-1-AP
|Click here to view our Standard Protocols
Free Radic Biol Med
NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.
Behav Brain Res
Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running.
Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors.
Absence of BBSome function leads to astrocyte reactivity in the brain.
L116 Deletion in CSPα Promotes α-Synuclein Aggregation and Neurodegeneration
Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function
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julie (Verified Customer) (03-28-2022)