CPT1A Polyclonal antibody

• Featured Product
• KD/KO Validated

CPT1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

226

Reviews (2)

Cat no : 15184-1-AP

Synonyms

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

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Validation Data Gallery

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  WB analysis of A549 cells using 15184-1-AP

  WB results of CPT1A antibody (1:1000) with shCPT1A-A549 and shCPT1A-HepG2 cells. shControl-A549 and shControl-HepG2 as positive control.

• 

  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15184-1-AP

  IP result of anti-CPT1A (IP:15184-1-AP, 4ug; Detection:15184-1-AP 1:1000) with HepG2 cells lysate 3000 ug.

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  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP (CPT1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15184-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15184-1-AP (CPT1A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15184-1-AP

  Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells stained for CPT1A rabbit pAb (15184-1-AP) and KRT18 mouse mAb (66187-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15184-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

• 

  FC experiment of HeLa using 15184-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CPT1A (15184-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HepG2 cells, MCF-7 cells, mouse brain tissue, mouse lung tissue, rat brain tissue, rat lung tissue, mouse liver tissue, rat liver tissue, U-251 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 15 publications below
• WB: See 207 publications below
• IHC: See 23 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• FC: See 4 publications below

Product Information

15184-1-AP targets CPT1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, pig, yellow croaker, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPT1A fusion protein Ag7202
• Full Name: carnitine palmitoyltransferase 1A (liver)
• Calculated molecular weight: 88 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC000185
• Gene symbol: CPT1A
• Gene ID (NCBI): 1374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Publications

Species	Application	Title
mouse	WB	Nature; High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.; Authors - Semir Beyaz
mouse	WB,FC	Cell; Mitochondrial Priming by CD28.; Authors - Ramon I Klein Geltink ; KD Validated
mouse	IHC	Cell Metab; SGLT2 Inhibition Mediates Protection from Diabetic Kidney Disease by Promoting Ketone Body-Induced mTORC1 Inhibition.; Authors - Issei Tomita
		Cell Metab; Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity.; Authors - Mauro Corrado
human	WB	Nat Immunol; Foxp3 and Toll-like receptor signaling balance Treg cell anabolic metabolism for suppression.; Authors - Valerie A Gerriets
human	WB	Nat Commun; Metabolic reprogramming from glycolysis to fatty acid uptake and beta-oxidation in platinum-resistant cancer cells.; Authors - Yuying Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (05-17-2021)

Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

CPT1A Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:15184-1-AP)

FH

Linlin (Verified Customer) (05-08-2019)

This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 2ug per pulldown; 1000x for immunoblot analysis
• Cell Tissue Type: mice lung macrophages cell line

CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)

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