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  • KD/KO Validated

CPT1A Polyclonal antibody

CPT1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15184-1-AP

Synonyms

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1



Tested Applications

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, MCF-7 cells, mouse brain tissue, mouse lung tissue, rat brain tissue, rat lung tissue, mouse liver tissue, rat liver tissue, U-251 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15184-1-AP targets CPT1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, pig, yellow croaker, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPT1A fusion protein Ag7202
Full Name carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight 88 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC000185
Gene symbol CPT1A
Gene ID (NCBI) 1374
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Protocols

Product Specific Protocols
WB protocol for CPT1A antibody 15184-1-APDownload protocol
IHC protocol for CPT1A antibody 15184-1-APDownload protocol
IF protocol for CPT1A antibody 15184-1-APDownload protocol
IP protocol for CPT1A antibody 15184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.

Authors - Semir Beyaz
mouseWB,FC

Cell

Mitochondrial Priming by CD28.

Authors - Ramon I Klein Geltink
  • KD Validated
mouseIHC

Cell Metab

SGLT2 Inhibition Mediates Protection from Diabetic Kidney Disease by Promoting Ketone Body-Induced mTORC1 Inhibition.

Authors - Issei Tomita

Cell Metab

Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity.

Authors - Mauro Corrado
humanWB

Nat Immunol

Foxp3 and Toll-like receptor signaling balance Treg cell anabolic metabolism for suppression.

Authors - Valerie A Gerriets
humanWB

Nat Commun

Metabolic reprogramming from glycolysis to fatty acid uptake and beta-oxidation in platinum-resistant cancer cells.

Authors - Yuying Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (05-17-2021)

Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293
CPT1A Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:15184-1-AP)
FH

Linlin (Verified Customer) (05-08-2019)

This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 2ug per pulldown; 1000x for immunoblot analysis
  • Cell Tissue Type: mice lung macrophages cell line
CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)