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CPT1A Polyclonal antibody

CPT1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15184-1-AP

Synonyms

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1



Tested Applications

Positive WB detected inmouse liver tissue, A549 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15184-1-AP targets CPT1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity cows, human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPT1A fusion protein Ag7202
Full Name carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight 88 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC000185
Gene symbol CPT1A
Gene ID (NCBI) 1374
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Protocols

Product Specific Protocols
WB protocol for CPT1A antibody 15184-1-APDownload protocol
IHC protocol for CPT1A antibody 15184-1-APDownload protocol
IF protocol for CPT1A antibody 15184-1-APDownload protocol
IP protocol for CPT1A antibody 15184-1-APDownload protocol
FC protocol for CPT1A antibody 15184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.

Authors - Lina Zhou
humanWB

J Lipid Res

CD36 plays a negative role in the regulation of lipophagy in hepatocytes through an AMPK-dependent pathway.

Authors - Yun Li
humanWB

Food Funct

Coenzyme Q10 attenuates high-fat diet-induced non-alcoholic fatty liver disease through activation of the AMPK pathway.

Authors - Ke Chen
mouseWB

Am J Physiol Gastrointest Liver Physiol

The Role of Ketone Signaling in the Hepatic Response to Fasting.

Authors - Caroline E Geisler
mouseWB

Am J Physiol Gastrointest Liver Physiol

Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice.

Authors - Liu Yao
mouseWB

Physiol Rep

Adropin reduces blood glucose levels in mice by limiting hepatic glucose production.

Authors - Dharendra Thapa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Linlin (Verified Customer) (05-08-2019)

This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 2ug per pulldown; 1000x for immunoblot analysis
  • Cell Tissue Type: mice lung macrophages cell line
CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)