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CPT1A Polyclonal antibody
CPT1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
151
Cat no : 15184-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, A549 cells, HepG2 cells, MCF-7 cells, U-251 cells, mouse brain, rat brain |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, mouse brain tissue, human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, Hela cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 134 publications below |
| IHC | See 17 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| FC | See 3 publications below |
Product Information
15184-1-AP targets CPT1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | bovine, human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CPT1A fusion protein Ag7202 |
| Full Name | carnitine palmitoyltransferase 1A (liver) |
| Calculated molecular weight | 88 kDa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | BC000185 |
| Gene symbol | CPT1A |
| Gene ID (NCBI) | 1374 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CPT1A antibody 15184-1-AP | Download protocol |
| IHC protocol for CPT1A antibody 15184-1-AP | Download protocol |
| IF protocol for CPT1A antibody 15184-1-AP | Download protocol |
| IP protocol for CPT1A antibody 15184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Proteome Res Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation. | ||
J Lipid Res CD36 plays a negative role in the regulation of lipophagy in hepatocytes through an AMPK-dependent pathway. | ||
Food Funct Coenzyme Q10 attenuates high-fat diet-induced non-alcoholic fatty liver disease through activation of the AMPK pathway. | ||
Am J Physiol Gastrointest Liver Physiol The Role of Ketone Signaling in the Hepatic Response to Fasting. | ||
Am J Physiol Gastrointest Liver Physiol Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice. | ||
Physiol Rep Adropin reduces blood glucose levels in mice by limiting hepatic glucose production. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (05-17-2021) | Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.
 |
FH Linlin (Verified Customer) (05-08-2019) | This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.
 |
