CPT1A Polyclonal antibody

• Featured Product
• KD/KO Validated

CPT1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

196

Reviews (2)

Cat no : 15184-1-AP

Synonyms

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

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Validation Data Gallery

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  WB analysis of A549 cells using 15184-1-AP

  WB results of CPT1A antibody (1:1000) with shCPT1A-A549 and shCPT1A-HepG2 cells. shControl-A549 and shControl-HepG2 as positive control.

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  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15184-1-AP

  IP result of anti-CPT1A (IP:15184-1-AP, 4ug; Detection:15184-1-AP 1:1000) with HepG2 cells lysate 3000 ug.

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  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP (CPT1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP (CPT1A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15184-1-AP (CPT1A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15184-1-AP (CPT1A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15184-1-AP (CPT1A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15184-1-AP (CPT1A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15184-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15184-1-AP (CPT1A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15184-1-AP

  Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells stained for CPT1A rabbit pAb (15184-1-AP) and KRT18 mouse mAb (66187-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15184-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

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  IF Staining of HeLa using 15184-1-AP

  Immunofluorescent analysis of Hela cells, using CPT1A antibody 15184-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15184-1-AP

  1X10^6 HeLa cells were stained with 0.2ug CPT1A antibody (15184-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse liver tissue, A549 cells, HepG2 cells, MCF-7 cells, U-251 cells, mouse brain, rat brain
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, mouse brain tissue, human kidney tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, Hela cells
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 13 publications below
• WB: See 178 publications below
• IHC: See 21 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Product Information

15184-1-AP targets CPT1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, yellow croaker, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPT1A fusion protein Ag7202
• Full Name: carnitine palmitoyltransferase 1A (liver)
• Calculated molecular weight: 88 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC000185
• Gene symbol: CPT1A
• Gene ID (NCBI): 1374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Publications

Species	Application	Title
human	WB	J Proteome Res; Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.; Authors - Lina Zhou
human	WB	J Lipid Res; CD36 plays a negative role in the regulation of lipophagy in hepatocytes through an AMPK-dependent pathway.; Authors - Yun Li
human	WB	Food Funct; Coenzyme Q10 attenuates high-fat diet-induced non-alcoholic fatty liver disease through activation of the AMPK pathway.; Authors - Ke Chen
mouse	WB	Am J Physiol Gastrointest Liver Physiol; The Role of Ketone Signaling in the Hepatic Response to Fasting.; Authors - Caroline E Geisler
mouse	WB	Am J Physiol Gastrointest Liver Physiol; Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice.; Authors - Liu Yao
mouse	WB	Physiol Rep; Adropin reduces blood glucose levels in mice by limiting hepatic glucose production.; Authors - Dharendra Thapa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (05-17-2021)

Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

CPT1A Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:15184-1-AP)

FH

Linlin (Verified Customer) (05-08-2019)

This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 2ug per pulldown; 1000x for immunoblot analysis
• Cell Tissue Type: mice lung macrophages cell line

CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)

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