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CPT1A Antibody 87 Publications

Rabbit Polyclonal| Catalog number: 15184-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

CPT1A Antibody WB A549 cells 15184-1-AP

WB results of CPT1A antibody (1:1000) with shCPT1A-A549 and shCPT1A-HepG2 cells. shControl-A549 and shControl-HepG2 as positive control.

CPT1A Antibody WB mouse liver tissue 15184-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

CPT1A Antibody IF HepG2 cells 15184-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 15184-1-AP (CPT1A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CPT1A Antibody IP HepG2 cells 15184-1-AP

IP result of anti-CPT1A(IP:15184-1-AP, 4ug; Detection:15184-1-AP 1:1000) with HepG2 cells lysate 3000 ug

CPT1A Antibody IHC human liver cancer tissue 15184-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP(CPT1A antibody) at dilution of 1:100 (under 10x lens)

CPT1A Antibody IHC human liver cancer tissue 15184-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP(CPT1A antibody) at dilution of 1:100 (under 40x lens)

CPT1A Antibody IF HepG2 cells 15184-1-AP

Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells stained for CPT1A rabbit pAb (15184-1-AP) and KRT18 mouse mAb (66187-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15184-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

CPT1A Antibody FC HeLa cells 15184-1-AP

1X10^6 HeLa cells were stained with 0.2ug CPT1A antibody (15184-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse liver tissue, A549 cells, HepG2 cells, MCF-7 cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human liver cancer tissue, human colon tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CPT1A fusion protein Ag7202
Full name:
carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight:
88 kDa
Observed molecular weight:
86 kDa
GenBank accession number:
BC000185
Gene ID (NCBI):
1374
Gene symbol
CPT1A
Synonyms
CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

Background

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

CPT1A Rabbit Polyclonal antibody

Linlin (Verified Customer), 05-08-2019
Applications
Western Blot,Immunoprecipitation,
Primary Antibody Dilution
2ug per pulldown; 1000x for immunoblot analysis
CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)

CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)

Cell/Tissue Type
mice lung macrophages cell line
Lot Number
00056856
This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

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