CREB1 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 12208-1-AP

  • Print page
  • Download PDF

Be the first to review this product


-_-

Freight/Packing

Con: 43 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, rat, monkey

Positive WB detected in:
multi-cells/tissue, COS-7 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, mouse lung tissue, NIH/3T3 cells, rat brain tissue

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human thyroid tissue, human prostate cancer tissue

Positive IF detected in:
HEK-293 cells

Positive FC detected in:
HEK-293 cells

Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cAMP responsive element binding protein 1

Calculated molecular weight:
341aa,35 kDa

Observed molecular weight:
43-46 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CREB1

Synonyms
CREB, CREB 1, CREB1
Background

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).


Back
to top