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- KD/KO Validated
CREB1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12208-1-AP
CREB, CREB 1, CREB1
|Positive WB detected in||multi-cells/tissue, COS-7 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, mouse lung tissue, NIH/3T3 cells, rat brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human cervical cancer tissue, mouse brain tissue, human prostate cancer tissue, human thyroid tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12208-1-AP targets CREB1 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat, sea cucumbers|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CREB1 fusion protein Ag2852|
|Full Name||cAMP responsive element binding protein 1|
|Calculated molecular weight||341aa,35 kDa|
|Observed molecular weight||43-46 kDa|
|GenBank accession number||BC010636|
|Gene ID (NCBI)||1385|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).
|Product Specific Protocols|
|WB protocol for CREB1 antibody 12208-1-AP||Download protocol|
|IHC protocol for CREB1 antibody 12208-1-AP||Download protocol|
|IF protocol for CREB1 antibody 12208-1-AP||Download protocol|
|IP protocol for CREB1 antibody 12208-1-AP||Download protocol|
|FC protocol for CREB1 antibody 12208-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Analysis of the oncogene BRAF mutation and the correlation of the expression of wild-type BRAF and CREB1 in endometriosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Shogo (Verified Customer) (02-22-2019)
Approximately 50 mg of whole brain were applied to ChIP assay. Fragmented DNA was immunoprecipitated with the CREB antibody (250 ng) at 4 oC for overnight.