Tested Applications
| Positive WB detected in | HEK-293 cells, COS-7 cells, Calyculin A treated HEK-293 cells, HL-1 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, multi-cells/tissue, rat brain tissue, HeLa cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, WB result of FTO antibody (12208-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and CREB1 knockout HL-1 cells., PC-3 cells, HL-60 cells, A431 cells, MCF-7 cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human cervical cancer tissue, human prostate cancer tissue, human thyroid tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
| Positive ChIP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Chromatin immunoprecipitation (ChIP) | CHIP : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12208-1-AP targets CREB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse, rat, chicken, goat, sea cucumbers |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2852 Product name: Recombinant human CREB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC010636 Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD Predict reactive species |
| Full Name | cAMP responsive element binding protein 1 |
| Calculated Molecular Weight | 341 aa, 35 kDa |
| Observed Molecular Weight | 43-46 kDa |
| GenBank Accession Number | BC010636 |
| Gene Symbol | CREB1 |
| Gene ID (NCBI) | 1385 |
| RRID | AB_2245417 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16220 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CREB1 antibody 12208-1-AP | Download protocol |
| IHC protocol for CREB1 antibody 12208-1-AP | Download protocol |
| IP protocol for CREB1 antibody 12208-1-AP | Download protocol |
| WB protocol for CREB1 antibody 12208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.
| ||
Mol Cell LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation | ||
Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. | ||
Environ Int Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH MALLIKARJUNA (Verified Customer) (10-24-2025) | best for western blot
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FH Vignesh (Verified Customer) (10-03-2025) |
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FH Georgiana (Verified Customer) (01-10-2023) |
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FH Shogo (Verified Customer) (02-22-2019) | Approximately 50 mg of whole brain were applied to ChIP assay.Fragmented DNA was immunoprecipitated with the CREB antibody (250 ng) at 4 oC for overnight.
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