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  • KD/KO Validated

CREST Polyclonal antibody

CREST Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 12439-1-AP


CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1

Tested Applications

Positive WB detected inK-562 cells, mouse brain tissue, human heart tissue, COLO 320 cells, HeLa cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse testis tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12439-1-AP targets CREST in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREST fusion protein Ag3119
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Calculated Molecular Weight 396 aa, 43 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC034494
Gene Symbol CREST
Gene ID (NCBI) 26039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.


Product Specific Protocols
WB protocol for CREST antibody 12439-1-APDownload protocol
IHC protocol for CREST antibody 12439-1-APDownload protocol
IF protocol for CREST antibody 12439-1-APDownload protocol
IP protocol for CREST antibody 12439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


human, mouseIHC,CoIP

Nat Neurosci

Exome sequencing to identify de novo mutations in sporadic ALS trios.

Authors - Chesi Alessandra A

J Neurosci

Kinetic Analysis of npBAF to nBAF Switching Reveals Exchange of SS18 with CREST and Integration with Neural Developmental Pathways.

Authors - Staahl Brett T BT

Cancers (Basel)

Global Chromatin Changes Resulting from Single-Gene Inactivation-The Role of SMARCB1 in Malignant Rhabdoid Tumor.

Authors - Colin Kenny

J Neurosci

CREST in the Nucleus Accumbens Core Regulates Cocaine Conditioned Place Preference, Cocaine-Seeking Behavior, and Synaptic Plasticity.

Authors - Yasaman Alaghband

PLoS Genet

Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.

Authors - Sangeun Park

Hum Mol Genet

Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis.

Authors - Michael Tibshirani


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Aktan (Verified Customer) (12-24-2019)

The product is tested in prostate cancer cell lines. Used at the given dilution factor, in 5% BSA in PBST, o/n at 4C. Bands were detected using Licor system and Licor near IR secondary antibodies.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer cell lines
CREST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:12439-1-AP)