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- KD/KO Validated
CREST Polyclonal antibody
CREST Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 12439-1-AP
|Positive WB detected in||K-562 cells, COLO 320 cells, human heart tissue, HeLa cells, mouse brain tissue|
|Positive IP detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, mouse testis tissue|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12439-1-AP targets CREST in WB, IP, IHC, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Drosophila, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CREST fusion protein Ag3119|
|Full Name||synovial sarcoma translocation gene on chromosome 18-like 1|
|Calculated molecular weight||396 aa, 43 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC034494|
|Gene ID (NCBI)||26039|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.
|Product Specific Protocols|
|WB protocol for CREST antibody 12439-1-AP||Download protocol|
|IHC protocol for CREST antibody 12439-1-AP||Download protocol|
|IF protocol for CREST antibody 12439-1-AP||Download protocol|
|IP protocol for CREST antibody 12439-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.
Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling.
Histochem Cell Biol
Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes.
Calcium-responsive transactivator (CREST) protein shares a set of structural and functional traits with other proteins associated with amyotrophic lateral sclerosis.
Hum Mol Genet
Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis.
CREST in the Nucleus Accumbens Core Regulates Cocaine Conditioned Place Preference, Cocaine-Seeking Behavior, and Synaptic Plasticity.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Aktan (Verified Customer) (12-24-2019)
The product is tested in prostate cancer cell lines. Used at the given dilution factor, in 5% BSA in PBST, o/n at 4C. Bands were detected using Licor system and Licor near IR secondary antibodies.