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  • KD/KO Validated

CREST Polyclonal antibody

CREST Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 12439-1-AP

Synonyms

CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1



Tested Applications

Positive WB detected inK-562 cells, COLO 320 cells, human heart tissue, HeLa cells, mouse brain tissue
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse testis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12439-1-AP targets CREST in WB, IP, IHC, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Drosophila, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREST fusion protein Ag3119
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Calculated molecular weight 396 aa, 43 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC034494
Gene symbol SS18L1
Gene ID (NCBI) 26039
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.

Protocols

Product Specific Protocols
WB protocol for CREST antibody 12439-1-APDownload protocol
IHC protocol for CREST antibody 12439-1-APDownload protocol
IF protocol for CREST antibody 12439-1-APDownload protocol
IP protocol for CREST antibody 12439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
DrosophilaWB

PLoS Genet

Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.

Authors - Sangeun Park
mouseIF

Learn Mem

Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling.

Authors - Annie Vogel Ciernia
human,ratWB,IHC,IF

Histochem Cell Biol

Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes.

Authors - Ana Du
DrosophilaWB

Mol Neurodegener

Calcium-responsive transactivator (CREST) protein shares a set of structural and functional traits with other proteins associated with amyotrophic lateral sclerosis.

Authors - Michail S Kukharsky
mouseIF

Hum Mol Genet

Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis.

Authors - Michael Tibshirani
ratWB,IF

J Neurosci

CREST in the Nucleus Accumbens Core Regulates Cocaine Conditioned Place Preference, Cocaine-Seeking Behavior, and Synaptic Plasticity.

Authors - Yasaman Alaghband

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aktan (Verified Customer) (12-24-2019)

The product is tested in prostate cancer cell lines. Used at the given dilution factor, in 5% BSA in PBST, o/n at 4C. Bands were detected using Licor system and Licor near IR secondary antibodies.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer cell lines
CREST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:12439-1-AP)