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CoraLite®594-conjugated CREST Polyclonal antibody

CREST Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG


human, mouse, rat




CoraLite®594 Fluorescent Dye

Cat no : CL594-12439


CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

Immunofluorescence (IF)IF : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-12439 targets CREST in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREST fusion protein Ag3119
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Calculated Molecular Weight 396 aa, 43 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC034494
Gene Symbol CREST
Gene ID (NCBI) 26039
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.


Product Specific Protocols
IF protocol for CL594 CREST antibody CL594-12439Download protocol
Standard Protocols
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