CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10944-1-AP

Synonyms

Corticoliberin, CRF, CRH



Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10944-1-AP targets CRH/CRF in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Brandt’s voles, human, Microtus Ochrogaster, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRH/CRF fusion protein Ag1371
Full Name CRH/CRF
Calculated molecular weight 21 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC011031
Gene symbol CRH
Gene ID (NCBI) 1392
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

Protocols

Product Specific Protocols
WB protocol for CRH/CRF antibody 10944-1-APDownload protocol
IHC protocol for CRH/CRF antibody 10944-1-APDownload protocol
IF protocol for CRH/CRF antibody 10944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.

Authors - Mika Yamanaka-Takaichi
ratIHC

Anat Rec (Hoboken)

A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model.

Authors - Xiao-Juan Li
ratWB,IF

J Neurol Sci

Effects of chronic noise on mRNA and protein expression of CRF family molecules and its relationship with p-tau in the rat prefrontal cortex.

Authors - Zhihui Gai
ratIF

J Chem Neuroanat

PMID: 32315740

Authors - Li Zhou
mouseIHC,IF

Gen Comp Endocrinol

Expression of stress hormones AVP and CRH in the hypothalamus of Mus musculus following water and food deprivation.

Authors - Arun Kumar Yadawa
ratWB,IHC

Int J Mol Med

Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia.

Authors - Chunyang Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

roberto (Verified Customer) (06-07-2019)

High specific signal

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: postnatal mouse brain
CRH/CRF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in postnatal mouse brain (Cat no:10944-1-AP)
FH

Yan (Verified Customer) (10-31-2017)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: human postmortem brain
CRH/CRF Antibody Western Blot validation (1:10000 dilution) in human postmortem brain (Cat no:10944-1-AP)