CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)





Cat no : 10944-1-AP


Corticoliberin, CRF, CRH

Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10944-1-AP targets CRH/CRF in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Microtus Ochrogaster, zebrafish, Brandt’s voles, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRH/CRF fusion protein Ag1371
Full Name CRH/CRF
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC011031
Gene Symbol CRH
Gene ID (NCBI) 1392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.


Product Specific Protocols
WB protocol for CRH/CRF antibody 10944-1-APDownload protocol
IHC protocol for CRH/CRF antibody 10944-1-APDownload protocol
IF protocol for CRH/CRF antibody 10944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


Microtus OchrogasterWB

Neurobiol Stress

Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles.

Authors - Meghan Donovan


Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.

Authors - Xuebin Zhang

Brain Behav Immun

Defects in NLRP6, autophagy and goblet cell homeostasis are associated with reduced duodenal CRH receptor 2 expression in patients with functional dyspepsia.

Authors - Jessica K Bruce

Int J Mol Sci

Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.

Authors - Mika Yamanaka-Takaichi


PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons.

Authors - Roberto Oleari

Chin Med

Electroacupuncture pretreatment mediates sympathetic nerves to alleviate myocardial ischemia-reperfusion injury via CRH neurons in the paraventricular nucleus of the hypothalamus

Authors - Jie Zhou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


roberto (Verified Customer) (06-07-2019)

High specific signal

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: postnatal mouse brain
CRH/CRF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in postnatal mouse brain (Cat no:10944-1-AP)

Yan (Verified Customer) (10-31-2017)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: human postmortem brain
CRH/CRF Antibody Western Blot validation (1:10000 dilution) in human postmortem brain (Cat no:10944-1-AP)