Tested Applications
| Positive WB detected in | human brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 16 publications below |
| IHC | See 12 publications below |
| IF | See 16 publications below |
Product Information
10944-1-AP targets CRH/CRF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish, brandt’s voles, microtus ochrogaster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CRH/CRF fusion protein Ag1371 Predict reactive species |
| Full Name | CRH/CRF |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 35-38 kDa |
| GenBank Accession Number | BC011031 |
| Gene Symbol | CRH |
| Gene ID (NCBI) | 1392 |
| RRID | AB_2084279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06850 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRH/CRF antibody 10944-1-AP | Download protocol |
| IHC protocol for CRH/CRF antibody 10944-1-AP | Download protocol |
| IF protocol for CRH/CRF antibody 10944-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurobiol Stress Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles. | ||
Elife Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma. | ||
Brain Behav Immun Defects in NLRP6, autophagy and goblet cell homeostasis are associated with reduced duodenal CRH receptor 2 expression in patients with functional dyspepsia. | ||
Int J Mol Sci Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. | ||
Development PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons. | ||
J Ethnopharmacol Antidepressant and Anxiolytic Effects of Wuling Capsule in CSDS Mice: Alleviating HPA Axis Hyperactivity via the Nesfatin-1/NF-κB Signaling Pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH roberto (Verified Customer) (06-07-2019) | High specific signal
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FH Yan (Verified Customer) (10-31-2017) |
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