Validation Data Gallery
|Positive WB detected in||human brain tissue, rat brain tissue|
|Positive IHC detected in||human pancreas cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10944-1-AP targets CRH/CRF in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Microtus Ochrogaster, zebrafish, Brandt’s voles, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CRH/CRF fusion protein Ag1371|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC011031|
|Gene ID (NCBI)||1392|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.
Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles.
Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.
Brain Behav Immun
Defects in NLRP6, autophagy and goblet cell homeostasis are associated with reduced duodenal CRH receptor 2 expression in patients with functional dyspepsia.
Int J Mol Sci
Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.
PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
roberto (Verified Customer) (06-07-2019)
High specific signal
Yan (Verified Customer) (10-31-2017)