|Positive WB detected in||human brain tissue, rat brain tissue|
|Positive IHC detected in||human pancreas cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10944-1-AP targets CRH/CRF in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Brandt’s voles, human, Microtus Ochrogaster, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CRH/CRF fusion protein Ag1371|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC011031|
|Gene ID (NCBI)||1392|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.
Int J Mol Sci
Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.
Anat Rec (Hoboken)
A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model.
J Neurol Sci
Effects of chronic noise on mRNA and protein expression of CRF family molecules and its relationship with p-tau in the rat prefrontal cortex.
J Chem Neuroanat
Gen Comp Endocrinol
Expression of stress hormones AVP and CRH in the hypothalamus of Mus musculus following water and food deprivation.
Int J Mol Med
Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
roberto (Verified Customer) (06-07-2019)
High specific signal
Yan (Verified Customer) (10-31-2017)