CRNN Polyclonal antibody

CRNN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11799-1-AP

Synonyms

58 kDa heat shock protein, C1orf10, cornulin, CRNN, DRC1, PDRC1, SEP53, Tumor related protein



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells
Positive IHC detected inhuman oesophagus tissue, human cervical cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11799-1-AP targets CRNN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRNN fusion protein Ag2374
Full Name cornulin
Calculated molecular weight 495 aa, 54 kDa
Observed molecular weight 70 kDa, 58 kDa
GenBank accession numberBC030807
Gene symbol CRNN
Gene ID (NCBI) 49860
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cornulin, also known as C1orf10, is a member of the "fused gene" family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. It may play a role in the mucosal/epithelial immune response and epidermal differentiation. Specific to squamous epithelia cells, cornulin is expressed in esophagus, primary keratinocytes, scalp skin, foreskin, cervix, larynx, and fetal bladder. It may be a marker of late epidermal differentiation, and is down-regulated in eczema.

Protocols

Product Specific Protocols
WB protocol for CRNN antibody 11799-1-APDownload protocol
IHC protocol for CRNN antibody 11799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Oral Investig

Downregulation of CRNN gene and genomic instability at 1q21.3 in oral squamous cell carcinoma.

Authors - Iman Salahshourifar
humanIHC

J Thorac Cardiovasc Surg

Loss of CRNN expression is associated with advanced tumor stage and poor survival in patients with esophageal squamous cell carcinoma.

Authors - Hsu Po-Kuei PK
humanWB,IHC

J Proteomics

Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma.

Authors - Katada Koji K