|Positive WB detected in||mouse brain tissue, HepG2 cells|
|Positive IHC detected in||human oesophagus tissue, human cervical cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11799-1-AP targets CRNN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CRNN fusion protein Ag2374|
|Calculated molecular weight||495 aa, 54 kDa|
|Observed molecular weight||70 kDa, 58 kDa|
|GenBank accession number||BC030807|
|Gene ID (NCBI)||49860|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cornulin, also known as C1orf10, is a member of the "fused gene" family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. It may play a role in the mucosal/epithelial immune response and epidermal differentiation. Specific to squamous epithelia cells, cornulin is expressed in esophagus, primary keratinocytes, scalp skin, foreskin, cervix, larynx, and fetal bladder. It may be a marker of late epidermal differentiation, and is down-regulated in eczema.
Clin Oral Investig
Downregulation of CRNN gene and genomic instability at 1q21.3 in oral squamous cell carcinoma.
J Thorac Cardiovasc Surg
Loss of CRNN expression is associated with advanced tumor stage and poor survival in patients with esophageal squamous cell carcinoma.
Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma.