CRNN Polyclonal antibody

• Featured Product
• KD/KO Validated

CRNN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 11799-1-AP

Synonyms

58 kDa heat shock protein, C1orf10, cornulin, CRNN, DRC1, PDRC1, SEP53, Tumor related protein

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Validation Data Gallery

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  WB analysis of mouse brain using 11799-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11799-1-AP (CRNN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11799-1-AP (CRNN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human cervical cancer using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11799-1-AP (CRNN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11799-1-AP (CRNN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200.

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11799-1-AP (CRNN Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11799-1-AP (CRNN Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells
• Positive IHC detected in: human oesophagus tissue, human cervical cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Product Information

11799-1-AP targets CRNN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRNN fusion protein Ag2374
• Full Name: cornulin
• Calculated Molecular Weight: 495 aa, 54 kDa
• Observed Molecular Weight: 70 kDa, 58 kDa
• GenBank Accession Number: BC030807
• Gene Symbol: CRNN
• Gene ID (NCBI): 49860
• RRID: AB_2085497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cornulin, also known as C1orf10, is a member of the "fused gene" family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. It may play a role in the mucosal/epithelial immune response and epidermal differentiation. Specific to squamous epithelia cells, cornulin is expressed in esophagus, primary keratinocytes, scalp skin, foreskin, cervix, larynx, and fetal bladder. It may be a marker of late epidermal differentiation, and is down-regulated in eczema.

Publications

Species	Application	Title
human,mouse	WB,IHC	J Invest Dermatol; Cornulin Is Induced in Psoriasis Lesions and Promotes Keratinocyte Proliferation via Phosphoinositide 3-Kinase/Akt Pathways.; Authors - Changji Li ; KD Validated
human	WB,IHC	J Proteomics; Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma.; Authors - Katada Koji K
human	IHC	Clin Oral Investig; Downregulation of CRNN gene and genomic instability at 1q21.3 in oral squamous cell carcinoma.; Authors - Iman Salahshourifar
human	IHC	J Thorac Cardiovasc Surg; Loss of CRNN expression is associated with advanced tumor stage and poor survival in patients with esophageal squamous cell carcinoma.; Authors - Hsu Po-Kuei PK
human	IHC	PLoS One; Expression of Ki-67, Cornulin and ISG15 in non-involved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC); Authors - Padmanabha Kumar Govindaraj
human	IHC	Ecancermedicalscience; Expression of cornulin in tongue squamous cell carcinoma.; Authors - Saira Saleem