TORC1 Polyclonal antibody

TORC1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10441-1-AP

Synonyms

CRTC1, KIAA0616, MECT1, TORC 1, TORC1, Transducer of CREB protein 1, WAMTP1



Tested Applications

Positive WB detected inmouse kidney tissue, A431 cells, Jurkat cells, L02 cells, mouse brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10441-1-AP targets TORC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TORC1 fusion protein Ag0693
Full Name CREB regulated transcription coactivator 1
Calculated molecular weight 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC028050
Gene symbol CRTC1
Gene ID (NCBI) 23373
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRTC1, also named as MECT1, TORC1, and WAMTP1, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. CRTC1 acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. It enhances the interaction of CREB1 with TAF4. CRTC1 regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. It is a potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. CRTC1 is also a coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, CRTC1 involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. It may be required for dendritic growth of developing cortical neurons. This is a rabbit polyclonal antibody raised against residues near N terminus of human CRTC1.

Protocols

Product Specific Protocols
WB protocol for TORC1 antibody 10441-1-APDownload protocol
IHC protocol for TORC1 antibody 10441-1-APDownload protocol
IP protocol for TORC1 antibody 10441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neural Regen Res

Hippocampal insulin resistance and the Sirtuin 1 signaling pathway in diabetes-induced cognitive dysfunction.

Authors - Hui Yang
ratIF

Front Mol Neurosci

Cell biological mechanisms of activity-dependent synapse to nucleus translocation of CRTC1 in neurons.

Authors - Toh Hean Ch'ng

Ann N Y Acad Sci

PMID: 26645136

Authors - Holly M Brown-Borg
mouseWB

Sci Rep

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Authors - Lei Wu