CRYZ Polyclonal antibody

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  WB analysis using 31629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31629-1-AP (CRYZ antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CRYZ Polyclonal Antibody for WB, ELISA

Cat No. 31629-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

QOR

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31629-1-AP targets CRYZ in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRYZ fusion protein Ag35066
• Full Name: crystallin, zeta (quinone reductase)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC039578
• Gene Symbol: CRYZ
• Gene ID (NCBI): 1429
• RRID: AB_3670055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q08257
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRYZ (Zeta-crystallin) also called quinone oxidoreductase and NADPH:quinone reductase, and it does not have alcohol dehydrogenase activity. It binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro) for CRYZ. It may act in the detoxification of xenobiotics. CRYZ interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species and enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding (PMID: 17497241; 20103721).