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- KD/KO Validated
Casein Kinase 1 Delta Polyclonal antibody
Casein Kinase 1 Delta Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14388-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, K-562 cells, HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
14388-1-AP targets Casein Kinase 1 Delta in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Casein Kinase 1 Delta fusion protein Ag5856 |
Full Name | casein kinase 1, delta |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC003558 |
Gene symbol | CSNK1D |
Gene ID (NCBI) | 1453 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
IF protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
IP protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions. | ||
Dev Cell Mitotic ER Exit Site Disassembly and Reassembly Are Regulated by the Phosphorylation Status of TANGO1. | ||
J Cell Biol CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.
| ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.
| ||
Cell Cycle Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression. |