Casein Kinase 1 Delta Polyclonal antibody

• Featured Product
• KD/KO Validated

Casein Kinase 1 Delta Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14388-1-AP

Synonyms

Casein Kinase 1 delta, casein kinase 1, delta, Casein kinase I isoform delta, CKI delta, CKId, CSNK1D, HCKID

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Validation Data Gallery

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  WB analysis of HeLa using 14388-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 delta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14388-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 delta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14388-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14388-1-AP

  IP Result of anti-Casein Kinase 1 delta (IP:14388-1-AP, 3ug; Detection:14388-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IF Staining of HepG2 using 14388-1-AP

  Immunofluorescent analysis of HepG2 cells, using CSNK1D antibody 14388-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14388-1-AP targets Casein Kinase 1 Delta in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Casein Kinase 1 Delta fusion protein Ag5856
• Full Name: casein kinase 1, delta
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC003558
• Gene symbol: CSNK1D
• Gene ID (NCBI): 1453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
		Mol Cancer; SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.; Authors - Qiwei Jiang
human	WB	Dev Cell; Mitotic ER Exit Site Disassembly and Reassembly Are Regulated by the Phosphorylation Status of TANGO1.; Authors - Miharu Maeda
human	WB	J Cell Biol; CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.; Authors - Xue Deng ; KD Validated
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu ; KD Validated
human	WB	Mol Cell Biol; DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.; Authors - Chen Hao H
mouse	WB	Cell Cycle; Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression.; Authors - Shu-Tao Qi