Casein Kinase 1 Delta Polyclonal antibody

Casein Kinase 1 Delta Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 14388-1-AP

Synonyms

Casein Kinase 1 delta, casein kinase 1, delta, Casein kinase I isoform delta, CKI delta, CKId, CSNK1D, HCKID



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14388-1-AP targets Casein Kinase 1 Delta in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Casein Kinase 1 Delta fusion protein Ag5856
Full Name casein kinase 1, delta
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC003558
Gene symbol CSNK1D
Gene ID (NCBI) 1453
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Casein Kinase 1 Delta antibody 14388-1-APDownload protocol
IF protocol for Casein Kinase 1 Delta antibody 14388-1-APDownload protocol
IP protocol for Casein Kinase 1 Delta antibody 14388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Pharmacol

Casein Kinase 1δ/ε Inhibitor, PF670462 Attenuates the Fibrogenic Effects of Transforming Growth Factor-β in Pulmonary Fibrosis.

Authors - Christine R Keenan
human

Front Cell Dev Biol

Casein Kinase 1 Family Member CK1δ/Hrr25 Is Required for Autophagosome Completion.

Authors - Yuting Li
mouseWB

Cell Cycle

Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression.

Authors - Shu-Tao Qi
humanWB

Mol Cell Biol

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Authors - Chen Hao H
humanWB

J Cell Biol

CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.

Authors - Xue Deng
  • KD Validated
humanWB

Dev Cell

Mitotic ER Exit Site Disassembly and Reassembly Are Regulated by the Phosphorylation Status of TANGO1.

Authors - Miharu Maeda