CTAGE5 Recombinant antibody, PBS Only

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  WB analysis using 83017-2-RR (same clone as 83017-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83017-2-RR (CTAGE5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83017-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83017-2-RR (same clone as 83017-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTAGE5 antibody (83017-2-RR, Clone: 230262F2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83017-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83017-2-RR (same clone as 83017-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CTAGE5 Recombinant antibody (83017-2-RR, Clone:230262F2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83017-2-PBS in a different storage buffer formulation.

CTAGE5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83017-2-PBS

Clone No.230262F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor, cTAGE family member 5

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Product Information

83017-2-PBS targets CTAGE5 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: CTAGE family, member 5
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_005930
• Gene Symbol: CTAGE5
• Gene ID (NCBI): 4253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96PC5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241).