CTAGE5 Recombinant antibody

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  WB analysis using 83017-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83017-2-RR (CTAGE5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83017-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTAGE5 antibody (83017-2-RR, Clone: 230262F2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 83017-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CTAGE5 Recombinant antibody (83017-2-RR, Clone:230262F2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CTAGE5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83017-2-RR

Clone No.230262F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor, cTAGE family member 5

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Tested Applications

• Positive WB detected in: U-87 MG cells, HEK-293T cells, PC-3 cells, HeLa cells, Jurkat cells
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83017-2-RR targets CTAGE5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: CTAGE family, member 5
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_005930
• Gene Symbol: CTAGE5
• Gene ID (NCBI): 4253
• RRID: AB_3670760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96PC5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241).