CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C4B2

Cat no : 66995-1-Ig

Synonyms

BARS, C terminal binding protein 1, CTBP, CTBP1



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, A549 cells, Jurkat cells, HL-60 cells, NIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66995-1-Ig targets CTBP1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CTBP1 fusion protein Ag28567
Full Name C-terminal binding protein 1
Calculated molecular weight 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011655
Gene symbol CTBP1
Gene ID (NCBI) 1487
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Protocols

Product Specific Protocols
WB protocol for CTBP1 antibody 66995-1-IgDownload protocol
IHC protocol for CTBP1 antibody 66995-1-IgDownload protocol
IF protocol for CTBP1 antibody 66995-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols