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CoraLite®594-conjugated CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C4B2

Cat no : CL594-66995

Synonyms

BARS, C terminal binding protein 1, CTBP, CTBP1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CTBP1 Monoclonal antibody (CL594-66995)

    IF Staining of HepG2 using CL594-66995

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66995 (CTBP1 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated CTBP1 Monoclonal antibody (CL594-66995)

    FC experiment of HepG2 using CL594-66995

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CTBP1 (CL594-66995, Clone:1C4B2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66995 targets CTBP1 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CTBP1 fusion protein Ag28567
Full Name C-terminal binding protein 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC011655
Gene Symbol CTBP1
Gene ID (NCBI) 1487
RRIDAB_2883636
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Protocols

Product Specific Protocols
IF protocol for CL594 CTBP1 antibody CL594-66995Download protocol
FC protocol for CL594 CTBP1 antibody CL594-66995Download protocol
Standard Protocols
Click here to view our Standard Protocols