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- KD/KO Validated
CTGF Polyclonal antibody
CTGF Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (4)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 23936-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human gliomas tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 51 publications below |
| IHC | See 13 publications below |
| IF | See 6 publications below |
| ELISA | See 1 publications below |
Product Information
The immunogen of 23936-1-AP is CTGF Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | canine, human, mouse, pig, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CTGF fusion protein Ag21008 |
| Full Name | connective tissue growth factor |
| Calculated molecular weight | 349 aa, 38 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC087839 |
| Gene symbol | CTGF |
| Gene ID (NCBI) | 1490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CTGF, also known as CCN2 or connective tissue growth factor, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. In western blotting, there are three different forms of CTGF reported: monomeric forms at approximately 36-38 kDa, homodimeric forms at approximately 70 kDa, and lower molecular mass fragment forms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CTGF antibody 23936-1-AP | Download protocol |
| IHC protocol for CTGF antibody 23936-1-AP | Download protocol |
| IF protocol for CTGF antibody 23936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Cell Res CTGF regulates cyclic stretch-induced vascular smooth muscle cell proliferation via microRNA-19b-3p. | ||
Biomed Pharmacother Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2. | ||
Cells Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP. | ||
J Endocrinol SRT1720 retards renal fibrosis via inhibition of HIF1α /GLUT1 in diabetic nephropathy. | ||
EBioMedicine Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. | ||
