|Positive WB detected in||mouse heart tissue, mouse skeletal muscle tissue|
|Positive IHC detected in||human heart tissue, human gliomas tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23936-1-AP is CTGF Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||canine, human, mouse, pig, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CTGF fusion protein Ag21008|
|Full Name||connective tissue growth factor|
|Calculated molecular weight||349 aa, 38 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||BC087839|
|Gene ID (NCBI)||1490|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CTGF, also known as CCN2 or connective tissue growth factor, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. In western blotting, there are three different forms of CTGF reported: monomeric forms at approximately 36-38 kDa, homodimeric forms at approximately 70 kDa, and lower molecular mass fragment forms.
Exp Cell Res
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