CTGF Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 23936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23936-1-AP (CTGF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 23936-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 23936-1-AP (CTGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 23936-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 23936-1-AP (CTGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23936-1-AP (CTGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23936-1-AP (CTGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 23936-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23936-1-AP (CTGF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CTGF Polyclonal Antibody for ELISA

Cat No. 23936-1-AP

Publications(99)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, ELISA

Synonyms

IBP 8, Hypertrophic chondrocyte-specific protein 24, HCS24, Cellular communication network factor 2, CCN2

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Published Applications

• KD/KO: See 5 publications below
• WB: See 81 publications below
• IHC: See 24 publications below
• IF: See 12 publications below
• ELISA: See 1 publications below

Product Information

23936-1-AP targets CTGF in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, canine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTGF fusion protein Ag21008
• Full Name: connective tissue growth factor
• Calculated Molecular Weight: 349 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC087839
• Gene Symbol: CTGF
• Gene ID (NCBI): 1490
• RRID: AB_2736836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29279
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTGF, also known as CCN2 or connective tissue growth factor, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. In western blotting, there are three different forms of CTGF reported: monomeric forms at approximately 36-38 kDa, homodimeric forms at approximately 70 kDa, and lower molecular mass fragment forms.

Publications

Species	Application	Title
mouse	WB	Redox Biol; Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor PAI-1 and contributes to lung fibrosis; Authors - Xiangqi Chen
human	IHC	EBioMedicine; Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.; Authors - Tianjiao Wang
human	IHC	Cell Death Differ; O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression.; Authors - Ying-Ying Liu ; KD Validated
human	WB	Oncogene; Deacetylation of tumor-suppressor MST1 in Hippo pathway induces its degradation through HBXIP-elevated HDAC6 in promotion of breast cancer growth.; Authors - L Li
rat	WB	Theranostics; Crucial Role of miR-433 in Regulating Cardiac Fibrosis.; Authors - Lichan Tao
mouse	WB	Acta Pharmacol Sin; KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury.; Authors - Dan Xu