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CTGF Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 23936-1-AP

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Tested Applications
Applications:
ELISA, IF, IHC, WB
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Images See all

23936-1-AP;mouse heart tissue

mouse heart tissue were subjected to SDS PAGE followed by western blot with 23936-1-AP( CTGF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

23936-1-AP;Transfected HEK-293 cells

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 23936-1-AP( CTGF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

23936-1-AP;human colon cancer tissue

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 23936-1-AP( CTGF Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

23936-1-AP;human colon cancer tissue

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 23936-1-AP( CTGF Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

23936-1-AP;HepG2 cells

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 23936-1-AP(CTGF antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, human; dog; miniping;rat; sheep; buffloa; pig, rat
Positive WB detected in:
mouse heart tissue, Transfected HEK-293 cells
Positive IHC detected in:
human colon cancer tissue, human colon cancer tissue, human gliomas tissue, human heart tissue, human hepatocirrhosis tissue
Positive IF detected in:
HepG2 cells, HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen Affinity purified
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CTGF fusion protein ag21008
Full name:
connective tissue growth factor
Calculated molecular weight:
349aa,38 kDa
Observed molecular weight:
38 kDa
GenBank accession number:
BC087839
Gene ID (NCBI):
1490
Gene symbol
CTGF
Synonyms
CCN family member 2, CCN2, CTGF, HCS24, IBP 8, IGF binding protein 8, IGFBP 8, IGFBP8, NOV2
Background

CTGF, also known as CCN2 or connective tissue growth factor, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. In western blotting, there are three different forms of CTGF reported: monomeric forms at approximately 36-38 kDa, homodimeric forms at approximately 70 kDa, and lower molecular mass fragment forms.


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