CTGF Polyclonal antibody

CTGF Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 25474-1-AP

Synonyms

CCN family member 2, CCN2, CTGF, HCS24, IBP 8, IGF binding protein 8, IGFBP 8, IGFBP8, NOV2



Tested Applications

Positive WB detected inHUVEC cells, HeLa cells, U2OS cells
Positive FC detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25474-1-AP targets CTGF in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, goat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name connective tissue growth factor
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberNM_001901
Gene Symbol CTGF
Gene ID (NCBI) 1490
RRIDAB_2918089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.

Protocols

Product Specific Protocols
WB protocol for CTGF antibody 25474-1-APDownload protocol
FC protocol for CTGF antibody 25474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.

Authors - Wen-Chih Liu
pigWB

Front Bioeng Biotechnol

Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis

Authors - Yuan Zhu
ratWB

Chin Med

Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis.

Authors - Wenchuan Qi
mouseWB,IF

J Agric Food Chem

Krill Oil Turns Off TGF-β1 Profibrotic Signaling in the Prevention of Diabetic Nephropathy

Authors - Xuechun Sun
human,mouseWB,IHC

Front Pharmacol

2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside ameliorates bleomycin-induced pulmonary fibrosis via regulating pro-fibrotic signaling pathways

Authors - Tsung-Teng Huang
mouseWB

Life Sci

Verteporfin inhibits the dedifferentiation of tubular epithelial cells via TGF-β1/Smad pathway but induces podocyte loss in diabetic nephropathy

Authors - Chenyang Qi