Tested Applications
| Positive WB detected in | HUVEC cells, HeLa cells, MG-63 cells, U2OS cells |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human ovary tumor tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25474-1-AP targets CTGF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, canine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | connective tissue growth factor |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_001901 |
| Gene Symbol | CTGF |
| Gene ID (NCBI) | 1490 |
| RRID | AB_2918089 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CTGF antibody 25474-1-AP | Download protocol |
| IP protocol for CTGF antibody 25474-1-AP | Download protocol |
| WB protocol for CTGF antibody 25474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CTGF/CCN2 antibody (Cat# 25474-1-AP) has 101 total citations published in journals including Nature Communications, EMBO Journal, FASEB Journal, Cell Death & Differentiation, Cell Death & Disease, Advanced Science, Molecular Medicine, Stem Cell Research & Therapy, and Phytomedicine. Associated research fields include fibrosis (cardiac, renal, hepatic, pulmonary), oncology (colorectal, gastric, hepatocellular, ovarian, melanoma), diabetic complications, Hippo/YAP and TGF-β signaling, wound healing, inflammation, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Nat Commun CD248 acts as a mechanosensory switch in fibroblast subsets to establish distinct pathological niches in renal fibrosis. | ||
Nat Commun Single-cell transcriptomics reveals hair growth retardation mediated by aberrant connective tissue sheath contraction in male androgenetic alopecia. | ||
J Nanobiotechnology Early assessment and treatment of ventricular remodeling in vivo via a targeted ultrasonic molecular probe loaded with oxygen and cholecystokinin | ||
J Nanobiotechnology Human adipose-derived mesenchymal stem cell-derived exosomes induce epithelial remodeling and anti-scar healing revealed by single-cell RNA sequencing | ||
MedComm (2020) Nucleosome assembly protein 1 like 1 (NAP1L1) promotes cardiac fibrosis by inhibiting YAP1 ubiquitination and degradation | ||
Adv Sci (Weinh) Integrative Multi-Omics Approaches Reveal Selectivity Profiles and Molecular Mechanisms of FIIN-2, a Covalent FGFR Inhibitor |
