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Gamma Cystathionase Polyclonal antibody

Gamma Cystathionase Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 12217-1-AP

Synonyms

CSE, CTH, Cystathionine gamma lyase, Gamma cystathionase



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, rat heart tissue, rat liver tissue, rat kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue, HepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12217-1-AP targets Gamma Cystathionase in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Cystathionase fusion protein Ag2872
Full Name cystathionase (cystathionine gamma-lyase)
Calculated Molecular Weight 405 aa, 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC015807
Gene Symbol CTH
Gene ID (NCBI) 1491
RRIDAB_2087497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.

Protocols

Product Specific Protocols
WB protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
IHC protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
IF protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
IP protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCell treatment

Cell Metab

Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer

Authors - Hannah N Bell
humanWB

Cell Metab

Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.

Authors - Jiajun Zhu
mouseWB

Cell Metab

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.

Authors - Saara Forsström
mouseWB

Cell Metab

mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.

Authors - Nahid A Khan
WB

Cell Metab

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Authors - Joni Nikkanen
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Piruthivi (Verified Customer) (12-09-2019)

Western blot was very clear.

  • Applications: Western Blot,