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Cathepsin B Polyclonal antibody

Cathepsin B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (4)





Cat no : 12216-1-AP


APP secretase, APPS, cathepsin B, Cathepsin B1, CPSB, CTSB

Tested Applications

Positive WB detected inA375 cells, HepG2 cells, HT-1080 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12216-1-AP targets Cathepsin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, frog
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin B fusion protein Ag2898
Full Name cathepsin B
Calculated molecular weight 339 aa, 38 kDa
Observed molecular weight 38 kDa, 44 kDa
GenBank accession numberBC010240
Gene symbol CTSB
Gene ID (NCBI) 1508
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).


Product Specific Protocols
WB protocol for Cathepsin B antibody 12216-1-APDownload protocol
IHC protocol for Cathepsin B antibody 12216-1-APDownload protocol
IF protocol for Cathepsin B antibody 12216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Weihao (Verified Customer) (02-03-2022)

Works well for mouse BMDM and human macrophages, recommend it

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500