Cathepsin B Polyclonal antibody

• Home
• Products
• Primary Antibodies
• Cathepsin B Polyclonal antibody (12216-1-AP)

• Featured Product
• KD/KO Validated

Cathepsin B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12216-1-AP

Publications(128)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain

• 

  WB analysis using 12216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12216-1-AP (Cathepsin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 12216-1-AP

  WB result of Cathepsin B antibody (12216-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin B transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 12216-1-AP

  IP result of anti-Cathepsin B (IP:12216-1-AP, 4ug; Detection:12216-1-AP 1:3000) with HepG2 cells lysate 2000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A375 using 12216-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Cathepsin B antibody (12216-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 12216-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Cathepsin B (12216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, HT-1080 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12216-1-AP targets Cathepsin B in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, zebrafish, bovine, frog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2898

  Product name: Recombinant human Cathepsin B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-339 aa of BC010240

  Sequence: MWQLWASLCCLLVLANARSRPSFHPVSDELVNYVNKRNTTWQAGHNFYNVDMGYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI

• Full Name: cathepsin B
• Calculated Molecular Weight: 339 aa, 38 kDa
• Observed Molecular Weight: 25 kDa, 31 kDa, 43 kDa
• GenBank Accession Number: BC010240
• Gene Symbol: Cathepsin B
• Gene ID (NCBI): 1508
• RRID: AB_2086929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07858
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Nature; Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.; Authors - Jiasheng Zhang
		Science; Atlas of lysosomal aging reveals a metabolite signature shared with lysosomal storage disorders.; Authors - Anna M. Puszynska
		Immunity; Repression of RIPK1 kinase by INPP5D inhibits expression of diverse proinflammatory mediators and late-onset Alzheimer's disease risk factors; Authors - Xingxing Xie
human	WB	Drug Resist Updat; TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4; Authors - Zhiwei He
mouse	WB,IF	Autophagy; LAMP2-FLOT2 interaction enhances autophagosome-lysosome fusion to protect the septic heart in response to ILC2; Authors - Rongjiao Shao
mouse	WB	Autophagy; The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.; Authors - Diya Wang

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

Weihao (Verified Customer) (02-03-2022)

Works well for mouse BMDM and human macrophages, recommend it

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500