• Featured Product
  • KD/KO Validated

Cathepsin B Polyclonal antibody

Cathepsin B Polyclonal Antibody for WB, IF, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IF, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 12216-1-AP

Synonyms

CTSB, Cathepsin B1, Cathepsin B light chain, Cathepsin B heavy chain, Cat B



Tested Applications

Positive WB detected inA375 cells, HepG2 cells, HT-1080 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12216-1-AP targets Cathepsin B in WB, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, frog, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin B fusion protein Ag2898
Full Name cathepsin B
Calculated Molecular Weight 339 aa, 38 kDa
Observed Molecular Weight 38 kDa, 44 kDa
GenBank Accession NumberBC010240
Gene Symbol Cathepsin B
Gene ID (NCBI) 1508
RRIDAB_2086929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

Protocols

Product Specific Protocols
WB protocol for Cathepsin B antibody 12216-1-APDownload protocol
IHC protocol for Cathepsin B antibody 12216-1-APDownload protocol
IF protocol for Cathepsin B antibody 12216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nature

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

Authors - Jiasheng Zhang
humanIF

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
mouseWB,IHC

Aging Cell

Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy

Authors - Jian Sun
humanWB

Redox Biol

Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.

Authors - Shumin Ouyang
humanWB

Sci Total Environ

TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles

Authors - Xingyao Pei
frogWB

Proc Natl Acad Sci U S A

Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.

Authors - Yang Xiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Weihao (Verified Customer) (02-03-2022)

Works well for mouse BMDM and human macrophages, recommend it

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500