Cathepsin K Polyclonal antibody

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  WB analysis using 11239-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11239-1-AP (Cathepsin K antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of ROS1728 using 11239-1-AP

  IP result of anti-Cathepsin K (IP:11239-1-AP, 3ug; Detection:11239-1-AP 1:500) with ROS1728 cells lysate 3200ug.

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  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11239-1-AP (Cathepsin K antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human osteosarcoma using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100.

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  IHC staining of human osteosarcoma using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100.

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  IF Staining of mouse brain using 11239-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 11239-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of ROS1728 using 11239-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using Cathepsin K antibody (11239-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

Cathepsin K Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 11239-1-AP

Publications(91)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

CTSK, EC:3.4.22.38, CTSO2, CTSO, catK

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Tested Applications

• Positive WB detected in: A549 cells, U-87 MG cells, ROS1728 cells
• Positive IP detected in: ROS1728 cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue, human osteosarcoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 14 publications below
• IF: See 16 publications below

Product Information

11239-1-AP targets Cathepsin K in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cathepsin K fusion protein Ag1761
• Full Name: cathepsin K
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 27 kDa, 38~46 kDa
• GenBank Accession Number: BC016058
• Gene Symbol: Cathepsin K
• Gene ID (NCBI): 1513
• RRID: AB_2245581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43235
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSK(cathepsin K), also named CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts (PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot (PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Circadian control of the secretory pathway maintains collagen homeostasis.; Authors - Joan Chang
mouse	IHC,IF	Biomaterials; BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts.; Authors - Yamei Xu
mouse	IF	Dev Cell; Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption; Authors - Mobai Li
mouse		ACS Appl Mater Interfaces; Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis; Authors - Wenzheng Liao
mouse		Br J Pharmacol; Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis.; Authors - Tongling Huang
mouse	IHC	Arthritis Rheum; Osteoarthritic change is delayed in a Ctsk-knockout mouse model of osteoarthritis.; Authors - Kozawa Eiji E

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isabel (Verified Customer) (07-03-2020)

Antibody worked really well to detect Cathepsin K in protein from mouse osteoclasts. Blot was really clean with no other bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary mouse osteoclasts

Cathepsin K Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:11239-1-AP)

FH

RICHA (Verified Customer) (10-09-2019)

Good but hard to get the signal

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

Cathepsin K Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:11239-1-AP)