Cathepsin K Polyclonal antibody

Cathepsin K Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11239-1-AP

Synonyms

Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X, CTS02, CTSK, CTSO, CTSO1, CTSO2, PKND, PYCD



Tested Applications

Positive IP detected inROS1728 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human osteosarcoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inROS1728 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11239-1-AP targets Cathepsin K in IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin K fusion protein Ag1761
Full Name cathepsin K
Calculated molecular weight 39 kDa
Observed molecular weight 29-36 kDa
GenBank accession numberBC016058
Gene symbol CTSK
Gene ID (NCBI) 1513
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSK(cathepsin K), also named as CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts(PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot(PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Protocols

Product Specific Protocols
IHC protocol for Cathepsin K antibody 11239-1-APDownload protocol
IF protocol for Cathepsin K antibody 11239-1-APDownload protocol
IP protocol for Cathepsin K antibody 11239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Theranostics

ClC-7 Regulates the Pattern and Early Development of Craniofacial Bone and Tooth.

Authors - Yanli Zhang
mouseWB

Sci Rep

Sequential, but not Concurrent, Incubation of Cathepsin K and L with Type I Collagen Results in Extended Proteolysis.

Authors - Akia N Parks
ratWB

J Bone Miner Metab

miR-142-5p promotes the osteoclast differentiation of bone marrow-derived macrophages via PTEN/PI3K/AKT/FoxO1 pathway.

Authors - Zhenkai Lou
mouseWB

Mol Med Rep

Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species.

Authors - Ying Pi
mouseWB

Med Sci Monit

Fluid Shear Stress Suppresses Osteoclast Differentiation in RAW264.7 Cells through Extracellular Signal-Regulated Kinase 5 (ERK5) Signaling Pathway.

Authors - Chongwen Ma
humanIHC

Osteoporos Int

Bone histomorphometry in a long-term hemodialysis patient with hypoparathyroidism and sarcoidosis.

Authors - K Sumida

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Isabel (Verified Customer) (07-03-2020)

Antibody worked really well to detect Cathepsin K in protein from mouse osteoclasts. Blot was really clean with no other bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Primary mouse osteoclasts
Cathepsin K Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:11239-1-AP)
FH

RICHA (Verified Customer) (10-09-2019)

Good but hard to get the signal

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Protein from mouse tail tendon
Cathepsin K Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:11239-1-AP)