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Cathepsin K Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 11239-1-AP

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Con: 55 μg/150 μl

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Tested Applications

Applications:
IP, IHC, IF, ELISA
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Images See all

Cathepsin K Antibody IP ROS1728 cells 11239-1-AP

IP Result of anti-Cathepsin K (IP:11239-1-AP, 3ug; Detection:11239-1-AP 1:500) with ROS1728 cells lysate 3200ug.

Cathepsin K Antibody IHC human breast cancer tissue 11239-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11239-1-AP( Cathepsin K antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cathepsin K Antibody IHC human breast cancer tissue 11239-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11239-1-AP( Cathepsin K antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cathepsin K Antibody IF ROS1728 cells 11239-1-AP

Immunofluorescent analysis of ROS1728 cells using 11239-1-AP(Cathepsin K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive IP detected in:
ROS1728 cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue, human osteosarcoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
ROS1728 cells, HepG2 cells
Recommended dilution:
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cathepsin K fusion protein Ag1761
Full name:
cathepsin K
Calculated molecular weight:
39 kDa
Observed molecular weight:
29-36 kDa
GenBank accession number:
BC016058
Gene ID (NCBI):
1513
Gene symbol
CTSK
Synonyms
Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X, CTS02, CTSK, CTSO, CTSO1, CTSO2, PKND, PYCD

Background

CTSK(cathepsin K), also named as CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts(PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot(PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).


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