Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HT-1080 cells, A549 cells, NIH/3T3 cells, MCF-7 cells, HeLa cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human colon tissue, mouse colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11381-1-AP targets Cortactin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cortactin fusion protein Ag1940|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||70-85 kDa|
|GenBank accession number||BC008799|
|Gene ID (NCBI)||2017|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).
|Product Specific Protocols|
|WB protocol for Cortactin antibody 11381-1-AP||Download protocol|
|IHC protocol for Cortactin antibody 11381-1-AP||Download protocol|
|IF protocol for Cortactin antibody 11381-1-AP||Download protocol|
|IP protocol for Cortactin antibody 11381-1-AP||Download protocol|
|FC protocol for Cortactin antibody 11381-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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