Cortactin Polyclonal antibody

Cortactin Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11381-1-AP


Amplaxin, cortactin, CTTN, EMS1, Oncogene EMS1, Src substrate cortactin

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HUVEC cells, HT-1080 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11381-1-AP targets Cortactin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cortactin fusion protein Ag1940
Full Name cortactin
Calculated Molecular Weight 57 kDa
Observed Molecular Weight70-85 kDa
GenBank Accession NumberBC008799
Gene Symbol CTTN
Gene ID (NCBI) 2017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).


Product Specific Protocols
WB protocol for Cortactin antibody 11381-1-APDownload protocol
IHC protocol for Cortactin antibody 11381-1-APDownload protocol
IF protocol for Cortactin antibody 11381-1-APDownload protocol
IP protocol for Cortactin antibody 11381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma

Genes Dev

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

Authors - Megan L Norris

Adv Healthc Mater

Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration

Authors - Zhao Wei


TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

Authors - Qichao Huang

Front Oncol

A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma

Authors - Hao Wang


Decreased expression of cortactin in the schizophrenia brain.

Authors - Hriday P Bhambhvani


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Angie (Verified Customer) (03-11-2024)

The antibody detected a band around 80 kD at dilution of 1:5000 incubated at room temperature for 2 hour followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: A549, HEK293, SHSY-5Y
Cortactin Antibody Western Blot validation (1:5000 dilution) in A549, HEK293, SHSY-5Y (Cat no:11381-1-AP)