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- KD/KO Validated
CUL4A-Specific Polyclonal antibody
CUL4A-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 14851-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 24 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
14851-1-AP targets CUL4A-Specific in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | cullin 4A |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 77-88 kDa |
| GenBank Accession Number | NM_003589 |
| Gene Symbol | CUL4A |
| Gene ID (NCBI) | 8451 |
| RRID | AB_2261175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody is specially against CUL4A,but not CUL4B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CUL4A-Specific antibody 14851-1-AP | Download protocol |
| IHC protocol for CUL4A-Specific antibody 14851-1-AP | Download protocol |
| FC protocol for CUL4A-Specific antibody 14851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy
| ||
Nat Immunol COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence. | ||
Mol Cell Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.
| ||
Dev Cell AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.
| ||
J Exp Clin Cancer Res PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer | ||
