CUL4A-Specific Polyclonal antibody

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  WB analysis using 14851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14851-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14851-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 14851-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CUL4A-Specific (14851-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

CUL4A-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 14851-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

CUL4A, cullin 4A, CUL-4A, CUL 4A

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 25 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

14851-1-AP targets CUL4A-Specific in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cullin 4A
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 77-88 kDa
• GenBank Accession Number: NM_003589
• Gene Symbol: CUL4A
• Gene ID (NCBI): 8451
• RRID: AB_2261175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13619
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody is specially against CUL4A,but not CUL4B.

Publications

Species	Application	Title
human	WB,IHC	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng ; KD Validated
mouse	WB	Nat Immunol; COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.; Authors - Menon Suchithra S
mouse	WB	Mol Cell; Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.; Authors - Peng Jiang ; KD Validated
human	WB	Dev Cell; AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.; Authors - Manuela Antonioli ; KD Validated
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
mouse	WB	Nat Commun; CSN6 drives carcinogenesis by positively regulating Myc stability.; Authors - Jian Chen