CXCL1 Polyclonal antibody

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  WB analysis using 12335-1-AP

  LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 12335-1-AP (CXCL1 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12335-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12335-1-AP (CXCL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12335-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12335-1-AP (CXCL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CXCL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12335-1-AP

Publications(74)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

C X C motif chemokine 1, C-X-C motif chemokine 1, CXCL 1, GRO-alpha(4-73), GRO-alpha(5-73)

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Tested Applications

• Positive WB detected in: LPS and Protein transport inhibitor treated HUVEC cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 40 publications below
• IF: See 21 publications below
• ELISA: See 3 publications below

Product Information

12335-1-AP targets CXCL1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL1 fusion protein Ag2917
• Full Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Calculated Molecular Weight: 8 kDa to 14 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC011976
• Gene Symbol: CXCL1
• Gene ID (NCBI): 2919
• RRID: AB_2087568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09341
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Publications

Species	Application	Title
human	WB	Cancer Cell; Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis; Authors - Jiang Chang
	IHC	Cell Host Microbe; Induction of bone loss by pathobiont-mediated Nod1 signaling in the oral cavity.; Authors - Jiao Yizu Y
human	IHC	J Exp Med; An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition.; Authors - Helen L Young
human	WB,IF	Cell Rep; Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer; Authors - Xinyu Yang ; KD Validated
	IHC	Cell Rep; Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models; Authors - Jiali Dong
	WB,IF	Cell Mol Life Sci; Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion; Authors - Shiju Ye