• Featured Product
  • KD/KO Validated

CXCL1 Polyclonal antibody

CXCL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12335-1-AP

Synonyms

C X C motif chemokine 1, CXCL1, FSP, GRO, GRO alpha(1 73), GRO1, GROa, Growth regulated alpha protein, MGSA, MGSA a, NAP 3, SCYB1



Tested Applications

Positive WB detected inLPS and Protein transport inhibitor treated HUVEC cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12335-1-AP targets CXCL1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL1 fusion protein Ag2917
Full Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Calculated molecular weight 8 kDa to 14 kDa
GenBank accession numberBC011976
Gene symbol CXCL1
Gene ID (NCBI) 2919
RRIDAB_2087568
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Protocols

Product Specific Protocols
WB protocol for CXCL1 antibody 12335-1-APDownload protocol
IHC protocol for CXCL1 antibody 12335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Host Microbe

Induction of bone loss by pathobiont-mediated Nod1 signaling in the oral cavity.

Authors - Jiao Yizu Y
humanIHC

J Exp Med

An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition.

Authors - Helen L Young
human,mouseIF

Cell Rep

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer

Authors - Xinyu Yang
  • KD Validated
mouseIHC

Cell Rep

Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models

Authors - Jiali Dong
mouseIF

Cell Mol Life Sci

Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion

Authors - Shiju Ye
human, mouseIHC

Kidney Int

Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo.

Authors - Ivica Grgic