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CXCL1 Polyclonal antibody

CXCL1 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF

Conjugate

Unconjugated

Cat no : 12335-1-AP

Synonyms

C X C motif chemokine 1, CXCL1, FSP, GRO, GRO alpha(1 73), GRO1, GROa, Growth regulated alpha protein, MGSA, MGSA a, NAP 3, SCYB1



Tested Applications

Positive IHC detected inhuman gliomas tissue, mouse liver tissue, human breast cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12335-1-AP targets CXCL1 in IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL1 fusion protein Ag2917
Full Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Calculated molecular weight 8 kDa to 14 kDa
GenBank accession numberBC011976
Gene symbol CXCL1
Gene ID (NCBI) 2919
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Protocols

Product Specific Protocols
IHC protocol for CXCL1 antibody 12335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

Maresin1 regulates neutrophil recruitment and IL-10 expression in Aspergillus fumigatus keratitis.

Authors - Qing Tang
humanWB,IF

Cell Death Dis

Cancer-associated fibroblasts enhance tumor-associated macrophages enrichment and suppress NK cells function in colorectal cancer.

Authors - Rongsheng Zhang
human,mouseWB,IHC,IF

Oncoimmunology

PMID: 29900050

Authors - Guohui Qin
humanIHC

Front Oncol

CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung Cancer.

Authors - Shengnan Yu
human,mouseWB,IHC,IF

Int J Mol Sci

The P72R Polymorphism in R248Q/W p53 Mutants Modifies the Mutant Effect on Epithelial to Mesenchymal Transition Phenotype and Cell Invasion via CXCL1 Expression.

Authors - Cristabelle De Souza
humanIHC

Clin Lab

IL-1 alpha regulates CXCL1, CXCL10 and ICAM1 in network form in oral keratinocytes.

Authors - Wu Tong T