CoraLite® Plus 488-conjugated CXCR4 Recombinant antibody

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  IF Staining of Jurkat using CL488-84904

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 CXCR4 antibody (CL488-84904, Clone: 241734B2 ) at dilution of 1:200.

CXCR4 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84904

Clone No.241734B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

241734B2, CD184, C-X-C chemokine receptor type 4, CXC R4, CXCR 4

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84904 targets CXCR4 in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-X-C motif) receptor 4
• Calculated Molecular Weight: 352 aa, 40 kDa
• GenBank Accession Number: BC020968
• Gene Symbol: CXCR4
• Gene ID (NCBI): 7852
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61073
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).