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Cytochrome c Polyclonal antibody
Cytochrome c Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (8)
WB, IHC, IF, FC, ELISA
Cat no : 10993-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, rat liver tissue, Jurkat cells, mouse kidney tissue, rat kidney tissue, C2C12 cells|
|Positive IHC detected in||human liver tissue, human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse eye tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 407 publications below|
|IHC||See 22 publications below|
|IF||See 19 publications below|
|FC||See 1 publications below|
10993-1-AP targets Cytochrome c in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, bovine, canine, chicken, goat, hamster, Hippospongia, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytochrome c fusion protein Ag1455|
|Full Name||cytochrome c, somatic|
|Calculated molecular weight||12 kDa|
|Observed molecular weight||12-15 kDa|
|GenBank accession number||BC009578|
|Gene ID (NCBI)||54205|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
|Product Specific Protocols|
|WB protocol for Cytochrome c antibody 10993-1-AP||Download protocol|
|IHC protocol for Cytochrome c antibody 10993-1-AP||Download protocol|
|IF protocol for Cytochrome c antibody 10993-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.
Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.
Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.
Newly synthesized AIFM1 determines the hypersensitivity of T lymphocytes to STING activation-induced cell apoptosis
Identification of an autoinhibitory, mitophagy-inducing peptide derived from the transmembrane domain of USP30.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kazu (Verified Customer) (12-14-2022)
Worked well for 4% PFA fixed mouse optic nerve using this antibody at dilution of 1:200. Little background observed.
Azita (Verified Customer) (06-02-2021)
Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000.
Ying (Verified Customer) (04-22-2021)
good antibody. work well with mouse cells
Chi (Verified Customer) (09-26-2019)
The antibody works very well with good signal and low background.
Xiaoping (Verified Customer) (10-22-2018)
The signal is good and the size is between 10-15kDa.