|Positive WB detected in||HeLa cells, HEK-293 cells, rat liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, mouse kidney tissue, HepG2 cells, C2C12 cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse eye tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10993-1-AP targets Cytochrome c in WB, IHC, IF, FC applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, chicken, goat, hamster, Hippospongia, human, mouse, pig, rat, rice|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytochrome c fusion protein Ag1455|
|Full Name||cytochrome c, somatic|
|Calculated molecular weight||12 kDa|
|Observed molecular weight||12-15 kDa|
|GenBank accession number||BC009578|
|Gene ID (NCBI)||54205|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice.
Am J Chin Med
Ferulic Acid Exerts Anti-apoptotic Effects against Ischemic Injury by Activating HSP70/Bcl-2- and HSP70/Autophagy-Mediated Signaling after Permanent Focal Cerebral Ischemia in Rats.
Neural Regen Res
Neuroprotective effects of rapamycin on spinal cord injury in rats by increasing autophagy and Akt signaling.
Photobiomodulation Preconditioning Prevents Cognitive Impairment in a Neonatal Rat Model of Hypoxia-ischemia.
Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-02-2021)
Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000.
Ying (Verified Customer) (04-22-2021)
good antibody. work well with mouse cells
Chi (Verified Customer) (09-26-2019)
The antibody works very well with good signal and low background.
Xiaoping (Verified Customer) (10-22-2018)
The signal is good and the size is between 10-15kDa.