CYP1A2-Specific Polyclonal antibody

CYP1A2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19936-1-AP

Synonyms

CP12, CP1A2, CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 1A2, Cytochrome P450 4, Cytochrome P450 P3, P3 450, P450(PA)



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.05 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19936-1-AP targets CYP1A2-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome P450, family 1, subfamily A, polypeptide 2
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_000761
Gene Symbol CYP1A2
Gene ID (NCBI) 1544
RRIDAB_10638909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

Protocols

Product Specific Protocols
WB protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IHC protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IF protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IP protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
FC protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Host Microbe

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Authors - Yunong Zeng
mouseIF

Hepatology

Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening.

Authors - Linjie Lv
humanIF

Acta Biomater

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Authors - Kun Du
humanIF

ACS Appl Mater Interfaces

Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip

Authors - Xiaofang Du
mouseWB

Br J Pharmacol

Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.

Authors - Li-Ping Jiang
mouseIF,WB

Cell Mol Gastroenterol Hepatol

Aryl Hydrocarbon Receptor Activity in Hepatocytes Sensitizes to Hyperacute Acetaminophen-Induced Hepatotoxicity in Mice.

Authors - Fenja A Schuran