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CYP1A2-Specific Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 19936-1-AP

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Con: 42 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

CYP1A2-Specific Antibody WB mouse liver tissue 19936-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP(CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CYP1A2-Specific Antibody IF Hela cells 19936-1-AP

Immunofluorescent analysis of Hela cells, using CYP1A2 antibody 19936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CYP1A2-Specific Antibody IP mouse liver tissue 19936-1-AP

IP Result of anti-CYP1A2-Specific (IP:19936-1-AP, 4ug; Detection:19936-1-AP 1:300) with mouse liver tissue lysate 4000ug.

CYP1A2-Specific Antibody IHC human liver tissue 19936-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 19936-1-AP( CYP1A2-Specific Antibody) at dilution of 1:200 (under 40x lens).

CYP1A2-Specific Antibody FC HeLa cells 19936-1-AP

1X10^6 HeLa cells were stained with .5ug CYP1A2-Specific antibody (19936-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse liver tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:400
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
cytochrome P450, family 1, subfamily A, polypeptide 2
Calculated molecular weight:
58 kDa
Observed molecular weight:
58 kDa
GenBank accession number:
NM_000761
Gene ID (NCBI):
1544
Gene symbol
CYP1A2
Synonyms
CP12, CP1A2, CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 1A2, Cytochrome P450 4, Cytochrome P450 P3, P3 450, P450(PA)

Background

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.


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