CYP1A2-Specific Polyclonal antibody
CYP1A2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 19936-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 9 publications below |
IF | See 2 publications below |
Product Information
19936-1-AP targets CYP1A2-Specific in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | cytochrome P450, family 1, subfamily A, polypeptide 2 |
Calculated molecular weight | 58 kDa |
Observed molecular weight | 58 kDa |
GenBank accession number | NM_000761 |
Gene symbol | CYP1A2 |
Gene ID (NCBI) | 1544 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.
Protocols
Product Specific Protocols | |
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WB protocol for CYP1A2-Specific antibody 19936-1-AP | Download protocol |
IHC protocol for CYP1A2-Specific antibody 19936-1-AP | Download protocol |
IF protocol for CYP1A2-Specific antibody 19936-1-AP | Download protocol |
IP protocol for CYP1A2-Specific antibody 19936-1-AP | Download protocol |
FC protocol for CYP1A2-Specific antibody 19936-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Pharmazie Different expression patterns of hepatic cytochrome P450 s during anaphylactic or lipopolysaccharide-induced inflammation. | ||
Exp Anim Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF- kB pathway. | ||
J Thorac Dis Decreased expression of hepatic cytochrome P450 1A2 (CYP1A2) in a chronic intermittent hypoxia mouse model. | ||
Sensors (Basel) Sensing the Generation of Intracellular Free Electrons Using the Inactive Catalytic Subunit of Cytochrome P450s as a Sink. |