CYP1A2-Specific Polyclonal antibody

CYP1A2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 19936-1-AP

Synonyms

CP12, CP1A2, CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 1A2, Cytochrome P450 4, Cytochrome P450 P3, P3 450, P450(PA)

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Validation Data Gallery

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  WB analysis of mouse liver using 19936-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 19936-1-AP

  Immunofluorescent analysis of Hela cells, using CYP1A2 antibody 19936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IP experiment of mouse liver using 19936-1-AP

  IP Result of anti-CYP1A2-Specific (IP:19936-1-AP, 4ug; Detection:19936-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 19936-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19936-1-AP (CYP1A2-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HeLa using 19936-1-AP

  1X10^6 HeLa cells were stained with .5ug CYP1A2-Specific antibody (19936-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• IF: See 2 publications below

Product Information

19936-1-AP targets CYP1A2-Specific in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 1, subfamily A, polypeptide 2
• Calculated molecular weight: 58 kDa
• Observed molecular weight: 58 kDa
• GenBank accession number: NM_000761
• Gene symbol: CYP1A2
• Gene ID (NCBI): 1544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

Publications

Species	Application	Title
human	WB	Eur J Pharm Sci; PMID: 30776468; Authors - Vinay R Sonawane
		Pharmazie; Different expression patterns of hepatic cytochrome P450 s during anaphylactic or lipopolysaccharide-induced inflammation.; Authors - N Moriya
mouse	WB	Exp Anim; Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF- kB pathway.; Authors - Bin Li
mouse	WB	J Thorac Dis; Decreased expression of hepatic cytochrome P450 1A2 (CYP1A2) in a chronic intermittent hypoxia mouse model.; Authors - Xiao-Bin Zhang
human	WB	Drug Des Devel Ther; Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.; Authors - Hongfang Li
		Sensors (Basel); Sensing the Generation of Intracellular Free Electrons Using the Inactive Catalytic Subunit of Cytochrome P450s as a Sink.; Authors - Damilare D Akintade