CYP1A2-Specific Polyclonal antibody

CYP1A2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19936-1-AP

Synonyms

CP12, CP1A2, CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 1A2, Cytochrome P450 4, Cytochrome P450 P3, P3 450, P450(PA)



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19936-1-AP targets CYP1A2-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome P450, family 1, subfamily A, polypeptide 2
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_000761
Gene symbol CYP1A2
Gene ID (NCBI) 1544
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

Protocols

Product Specific Protocols
WB protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IHC protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IF protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IP protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
FC protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Pharm Sci

PMID: 30776468

Authors - Vinay R Sonawane

Pharmazie

Different expression patterns of hepatic cytochrome P450 s during anaphylactic or lipopolysaccharide-induced inflammation.

Authors - N Moriya
mouseWB

Exp Anim

Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF- kB pathway.

Authors - Bin Li
mouseWB

J Thorac Dis

Decreased expression of hepatic cytochrome P450 1A2 (CYP1A2) in a chronic intermittent hypoxia mouse model.

Authors - Xiao-Bin Zhang

Sensors (Basel)

Sensing the Generation of Intracellular Free Electrons Using the Inactive Catalytic Subunit of Cytochrome P450s as a Sink.

Authors - Damilare D Akintade
mouseWB

Toxicol Sci

Hepatic, metabolic and toxicity evaluation of repeated oral administration of SnS2 nanoflowers in mice.

Authors - Disi Bai