Validation Data Gallery
|Positive WB detected in||human heart tissue, mouse heart tissue|
|Positive IHC detected in||human heart tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66376-1-Ig targets Cardiac Troponin I in WB, ISFET, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cardiac Troponin I fusion protein Ag16758|
|Full Name||troponin I type 3 (cardiac)|
|Calculated molecular weight||210 aa, 24 kDa|
|Observed molecular weight||26-28 kDa|
|GenBank accession number||BC096165|
|Gene ID (NCBI)||7137|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.
|Product Specific Protocols|
|WB protocol for Cardiac Troponin I antibody 66376-1-Ig||Download protocol|
|IHC protocol for Cardiac Troponin I antibody 66376-1-Ig||Download protocol|
|IF protocol for Cardiac Troponin I antibody 66376-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
KARTHIKEYAN (Verified Customer) (11-20-2019)
We used this antibody to stain HL-1 cardiomyocyte cells, and the antibody works really well.