Cardiac Troponin I Monoclonal antibody

Cardiac Troponin I Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig







Cat no : 66376-1-Ig


Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

Tested Applications

Positive WB detected inhuman heart tissue, rat heart tissue, mouse heart tissue, pig heart tissue, rabbit heart tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66376-1-Ig targets Cardiac Troponin I in WB, IF, IHC, ISFET, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cardiac Troponin I fusion protein Ag16758
Full Name troponin I type 3 (cardiac)
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC096165
Gene Symbol TNNI3
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.


Product Specific Protocols
WB protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
IHC protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
IF protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biosens Bioelectron

A handheld testing device for the fast and ultrasensitive recognition of cardiac troponin I via an ion-sensitive field-effect transistor

Authors - Yiqing Wang

Stem Cell Reports

Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue

Authors - Shi Shen

Stem Cell Res Ther

Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection.

Authors - Anan Huang

J Am Heart Assoc

Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.

Authors - Karthikeyan Thirugnanam

J Cell Mol Med

Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress.

Authors - Linmao Lyu

Front Pharmacol

Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway.

Authors - Hui Zheng


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


KARTHIKEYAN (Verified Customer) (11-20-2019)

We used this antibody to stain HL-1 cardiomyocyte cells, and the antibody works really well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100 for IF
  • Cell Tissue Type: Moue HL-1 Atrial cardiomyocytes
Cardiac Troponin I Antibody Immunofluorescence, validation (1:100 for IF dilution) in Moue HL-1 Atrial cardiomyocytes (Cat no:66376-1-Ig)