Cardiac Troponin I Monoclonal antibody

Cardiac Troponin I Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D5D6

Cat no : 66376-1-Ig

Synonyms

Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle



Tested Applications

Positive WB detected inhuman heart tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66376-1-Ig targets Cardiac Troponin I in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cardiac Troponin I fusion protein Ag16758
Full Name troponin I type 3 (cardiac)
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC096165
Gene symbol TNNI3
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
IHC protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
IF protocol for Cardiac Troponin I antibody 66376-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Exp Ther Med

Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.

Authors - Chandong Ding
mouseWB

Exp Physiol

DBA/1 mice display equivalent cardiac function to C57BL/6J mice.

Authors - Zhen Wang
mouseWB

J Cell Mol Med

Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress.

Authors - Linmao Lyu
mouseIF

J Am Heart Assoc

Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.

Authors - Karthikeyan Thirugnanam

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

We used this antibody to stain HL-1 cardiomyocyte cells, and the antibody works really well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100 for IF
  • Cell Tissue Type: Moue HL-1 Atrial cardiomyocytes
Cardiac Troponin I Antibody Immunofluorescence, validation (1:100 for IF dilution) in Moue HL-1 Atrial cardiomyocytes (Cat no:66376-1-Ig)