Cardiac Troponin I Monoclonal antibody

Cardiac Troponin I Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA, ISFET

Conjugate

Unconjugated

Publications

18

CloneNo.

1D5D6

Reviews (1)

Cat no : 66376-1-Ig

Synonyms

cTnI, TNNI3, 1D5D6

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Validation Data Gallery

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  WB analysis using 66376-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66376-1-Ig

  Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 66376-1-Ig

  Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66376-1-Ig

  Rat heart tissue were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin Iantibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens).

• 

  IHC staining of human heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens).

• 

  IHC staining of mouse heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 66376-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 66376-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, rat heart tissue, mouse heart tissue, pig heart tissue, rabbit heart tissue
• Positive IHC detected in: human heart tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 10 publications below

Product Information

66376-1-Ig targets Cardiac Troponin I in WB, IHC, IF-P, ELISA, ISFET applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cardiac Troponin I fusion protein Ag16758
• Full Name: troponin I type 3 (cardiac)
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC096165
• Gene Symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• RRID: AB_2881753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.

Publications

Species	Application	Title
		ACS Nano; Collagen-Targeting Self-Assembled Nanoprobes for Multimodal Molecular Imaging and Quantification of Myocardial Fibrosis in a Rat Model of Myocardial Infarction; Authors - Fang Li
human	ISFET	Biosens Bioelectron; A handheld testing device for the fast and ultrasensitive recognition of cardiac troponin I via an ion-sensitive field-effect transistor; Authors - Yiqing Wang
pig	WB	Stem Cell Reports; Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue; Authors - Shi Shen
human	IF	Stem Cell Res Ther; Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection.; Authors - Anan Huang
mouse	IF	J Am Heart Assoc; Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.; Authors - Karthikeyan Thirugnanam
mouse	WB	J Cell Mol Med; Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress.; Authors - Linmao Lyu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

We used this antibody to stain HL-1 cardiomyocyte cells, and the antibody works really well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100 for IF
• Cell Tissue Type: Moue HL-1 Atrial cardiomyocytes

Cardiac Troponin I Antibody Immunofluorescence, validation (1:100 for IF dilution) in Moue HL-1 Atrial cardiomyocytes (Cat no:66376-1-Ig)