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Cardiac Troponin I Monoclonal antibody, PBS Only (66376-1-PBS)

Filter: AllWBIHCIF-P

WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)
Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)
Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
WB analysis of rat heart using 66376-1-Ig (same clone as 66376-1-PBS)
Rat heart tissue were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin Iantibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)
Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

Cardiac Troponin I Monoclonal antibody, PBS Only

Name: Cardiac Troponin I Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 66376-1-PBS

Cardiac Troponin I Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66376-1-PBS

Clone No.1D5D6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

cTnI, 1D5D6, TNNI3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66376-1-PBS targets Cardiac Troponin I in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag16758 Product name: Recombinant human TNNI3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE Predict reactive species
Full Name	troponin I type 3 (cardiac)
Calculated Molecular Weight	210 aa, 24 kDa
Observed Molecular Weight	26-28 kDa
GenBank Accession Number	BC096165
Gene Symbol	Cardiac Troponin I
Gene ID (NCBI)	7137
RRID	AB_2881753
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P19429
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.