Caspase 9/p35/p10 Monoclonal antibody
Caspase 9/p35/p10 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 66169-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lymphoma tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66169-1-Ig targets Caspase 9/p35/p10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, sheep, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Caspase 9/p35/p10 fusion protein Ag20813|
|Full Name||caspase 9, apoptosis-related cysteine peptidase|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||46 kDa, 35 kDa|
|GenBank accession number||BC002452|
|Gene ID (NCBI)||842|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].
|Product Specific Protocols|
|WB protocol for Caspase 9/p35/p10 antibody 66169-1-Ig||Download protocol|
|IHC protocol for Caspase 9/p35/p10 antibody 66169-1-Ig||Download protocol|
|IF protocol for Caspase 9/p35/p10 antibody 66169-1-Ig||Download protocol|
|IP protocol for Caspase 9/p35/p10 antibody 66169-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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