Caspase 9/p35/p10 Monoclonal antibody
Caspase 9/p35/p10 Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1B7G2
Cat no : 66169-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 38 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
66169-1-Ig targets Caspase 9/p35/p10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Caspase 9/p35/p10 fusion protein Ag20813 |
| Full Name | caspase 9, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 46 kDa, 35 kDa |
| GenBank accession number | BC002452 |
| Gene symbol | CASP9 |
| Gene ID (NCBI) | 842 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| IHC protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| IF protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| IP protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma. | ||
World J Gastrointest Oncol KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma. | ||
Cell Death Dis A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells. | ||
J Agric Food Chem β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway. | ||
Acta Cir Bras Hydroxysafflor yellow A acutely attenuates blood-brain barrier permeability, oxidative stress, inflammation and apoptosis in traumatic brain injury in rats1. | ||
Oxid Med Cell Longev Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK. |
