Caspase 9/p35/p10 Monoclonal antibody

Caspase 9/p35/p10 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

88

CloneNo.

1B7G2

Cat no : 66169-1-Ig

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6

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Validation Data Gallery

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  WB analysis of Jurkat using 66169-1-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/p35/p10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66169-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 66169-1-Ig

  IP Result of anti-Caspase 9/p35/p10 (IP:66169-1-Ig, 5ug; Detection:66169-1-Ig 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of human lymphoma using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 66169-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66169-1-Ig(Caspase 9/p35/p10 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 86 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below

Product Information

66169-1-Ig targets Caspase 9/p35/p10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, sheep, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 9/p35/p10 fusion protein Ag20813
• Full Name: caspase 9, apoptosis-related cysteine peptidase
• Calculated molecular weight: 46 kDa
• Observed molecular weight: 46 kDa, 35 kDa
• GenBank accession number: BC002452
• Gene symbol: CASP9
• Gene ID (NCBI): 842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Publications

Species	Application	Title
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
mouse	WB	J Pineal Res; Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota.; Authors - Wenkai Ren
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
human	WB,IF	Chem Sci; Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.; Authors - Meng Liu
human	WB	J Hazard Mater; Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.; Authors - Jing Qin
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong

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