Caspase 9/p35/p10 Monoclonal antibody

Caspase 9/p35/p10 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat no : 66169-1-Ig

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66169-1-Ig targets Caspase 9/p35/p10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 9/p35/p10 fusion protein Ag20813
Full Name caspase 9, apoptosis-related cysteine peptidase
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 35 kDa
GenBank accession numberBC002452
Gene symbol CASP9
Gene ID (NCBI) 842
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Protocols

Product Specific Protocols
WB protocol for Caspase 9/p35/p10 antibody 66169-1-IgDownload protocol
IHC protocol for Caspase 9/p35/p10 antibody 66169-1-IgDownload protocol
IF protocol for Caspase 9/p35/p10 antibody 66169-1-IgDownload protocol
IP protocol for Caspase 9/p35/p10 antibody 66169-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang
mouseWB

J Pineal Res

Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota.

Authors - Wenkai Ren
humanWB

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
humanWB,IF

Chem Sci

Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.

Authors - Meng Liu
humanWB

J Hazard Mater

Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.

Authors - Jing Qin
humanWB

Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong